Stewart M G, Siucińska E, Kossut M, Davies H
Brain and Behaviour Research Group, Open University, Milton Keynes, UK.
Brain Res. 1993 Sep 10;621(2):331-8. doi: 10.1016/0006-8993(93)90123-5.
Whisker follicles were surgically ablated (lesioned) on two entire rows (B and C) of the left snout of two groups of Swiss mice, in the first 2 days after birth (neonatally lesioned) with the animals being allowed to survive for 4 weeks. In the second group at 8 weeks of age (adults), the whisker follicles of rows B and C were similarly lesioned and a survival period of 3 days allowed. Glutamate-immunoreactivity (Glu-IR) was examined in tangential sections of the first somatosensory (SI) 'barrel' cortex of these two groups (at which time it was also confirmed that the follicles had not regrown). In the neonatally lesioned mice, barrel rows B and C were more poorly defined in the right (experimental) hemisphere sections and a semi-quantitative study showed that there was a decrease in Glu-IR cell number (up to 41%) in rows B and C of the right hemisphere compared to the spared barrel rows (A and D). The loss appears to occur over almost the entire area of each deprived barrel rather than being confined to the sides or hollows. In contrast to neonatally lesioned animals, the barrels of the adult-lesioned mice appeared intact and visually similar in both the experimental (right) and control hemispheres (left). The only notable change in Glu-IR observed in the adult-lesioned animals was a decrease of 38% in the number of Glu-IR cells in the sides between the two deafferented rows of barrels (B and C), compared to the cell number between the unaffected barrels (D and E), a change also seen in the neonatally lesioned mice.(ABSTRACT TRUNCATED AT 250 WORDS)
在两组瑞士小鼠出生后的头两天（新生期损伤），对其左鼻的两整排（B和C排）触须毛囊进行手术切除（损伤），让动物存活4周。在第二组8周龄（成年）时，同样对B和C排的触须毛囊进行损伤，并给予3天的存活期。对这两组动物第一体感（SI）“桶状”皮层的切线切片进行谷氨酸免疫反应性（Glu-IR）检测（此时还证实毛囊未再生）。在新生期损伤的小鼠中，右侧（实验）半球切片中的B和C桶排定义较差，半定量研究表明，与未损伤的桶排（A和D）相比，右侧半球B和C排的Glu-IR细胞数量减少（高达41%）。这种损失似乎发生在每个被剥夺的桶状区域的几乎整个区域，而不是局限于边缘或凹陷处。与新生期损伤的动物不同，成年期损伤小鼠的桶状结构在实验（右侧）和对照半球（左侧）中看起来都完好无损且视觉上相似。在成年期损伤的动物中观察到的Glu-IR的唯一显著变化是，与未受影响的桶排（D和E）之间的细胞数量相比，两个去传入桶排（B和C）之间边缘的Glu-IR细胞数量减少了38%，新生期损伤的小鼠也出现了这种变化。（摘要截断于250字）
