Hopkins R M, Thompson R C, Hobbs R P, Lymbery A J, Villa N, Smithyman T M
Institute for Molecular Genetics and Animal Disease, Murdoch University, Australia.
Acta Trop. 1993 Aug;54(2):117-24. doi: 10.1016/0001-706x(93)90057-i.
In this study indirect immunofluorescence was performed on both live and fixed trophozoites to determine the level of variability in surface antigen expression between 14 Giardia duodenalis isolates, using a monoclonal antibody raised against the Portland 1 isolate (ATCC No. 30888). Subsets of antigen positive cells were detected in 13 isolates ranging in number from < 1% to 50% of the total population. The differences in antigen expression between 10 uncloned isolates did not correlate with genetic differences determined using isoenzyme analysis. Examination of four clones of the Portland 1 isolate showed that all of the progeny expressed surface antigen at significantly different levels to the parent.
在本研究中，使用针对波特兰1株（ATCC编号30888）制备的单克隆抗体，对活的和固定的滋养体进行间接免疫荧光，以确定14株十二指肠贾第鞭毛虫分离株之间表面抗原表达的变异程度。在13株分离株中检测到抗原阳性细胞亚群，其数量占总群体的比例从＜1%到50%不等。10株未克隆分离株之间抗原表达的差异与使用同工酶分析确定的遗传差异无关。对波特兰1株的四个克隆进行检测，结果显示所有子代表达表面抗原的水平与亲本有显著差异。
