Ogi K, Miyamoto Y, Masuda Y, Habata Y, Hosoya M, Ohtaki T, Masuo Y, Onda H, Fujino M
Discovery Research Laboratories I, Takeda Chemical Industries Ltd., Ibaraki, Japan.
Biochem Biophys Res Commun. 1993 Nov 15;196(3):1511-21. doi: 10.1006/bbrc.1993.2423.
A functional cDNA clone for a human pituitary adenylate cyclase activating polypeptide (PACAP) receptor was isolated from a human pituitary cDNA library. The cDNA encoded a polypeptide consisting of 525 amino acids with putative seven hydrophobic domains. Chinese hamster ovary (CHO)-K1 cells transfected with the cDNA specifically bound PACAP and mediated PACAP-triggered intracellular accumulation of cAMP, indicating that this cDNA encoded a functional human PACAP Type I receptor. This receptor was structurally related to the vasoactive intestinal peptide (VIP), secretin, calcitonin and parathyroid hormone receptors and is much more homologous to a rat PACAP receptor. Northern blot analysis revealed that PACAP receptor mRNAs were expressed mainly in the brain and widely distributed in the central nervous system.
从人垂体cDNA文库中分离出一种人类垂体腺苷酸环化酶激活多肽（PACAP）受体的功能性cDNA克隆。该cDNA编码一个由525个氨基酸组成的多肽，具有七个推定的疏水区。用该cDNA转染的中国仓鼠卵巢（CHO）-K1细胞特异性结合PACAP，并介导PACAP触发的细胞内cAMP积累，表明该cDNA编码一种功能性人类I型PACAP受体。该受体在结构上与血管活性肠肽（VIP）、促胰液素、降钙素和甲状旁腺激素受体相关，并且与大鼠PACAP受体的同源性更高。Northern印迹分析显示，PACAP受体mRNA主要在脑中表达，并广泛分布于中枢神经系统。
