Nurse P
Department of Biochemistry, University of Oxford, U.K.
Philos Trans R Soc Lond B Biol Sci. 1993 Sep 29;341(1298):449-54. doi: 10.1098/rstb.1993.0127.
Genetic analysis using the fission yeast has provided a powerful methodology to investigate the eukaryotic cell cycle and its control. The onset of M-phase in fission yeast is controlled by a regulatory gene network which activates the p34cdc2 protein kinase encoded by the cdc2+ gene. The coupling of M-phase to the completion of S-phase also works through p34cdc2. A similar network is operative in vertebrate cells. Future work will focus on the controls regulating onset of S-phase and on the mechanisms by which a cell duplicates itself in space during division.
利用裂殖酵母进行的遗传分析为研究真核细胞周期及其调控提供了一种强大的方法。裂殖酵母中M期的起始由一个调控基因网络控制，该网络激活由cdc2+基因编码的p34cdc2蛋白激酶。M期与S期完成的耦合也通过p34cdc2起作用。类似的网络在脊椎动物细胞中也发挥作用。未来的工作将集中在调控S期起始的控制机制以及细胞在分裂过程中如何在空间上进行自我复制的机制上。
