Hozawa K, Takasaka T
Department of Otolaryngology, Tohoku University School of Medicine, Sendai, Japan.
Acta Otolaryngol Suppl. 1993;506:14-7. doi: 10.3109/00016489309130232.
The distribution of sympathetic fibers and calcitonin gene related peptide (CGRP)-positive fibers was examined in the endolymphatic sac of 10 guinea pigs by using immunohistochemical techniques to demonstrate tyrosine hydroxylase (TH) and CGRP. A meshwork of TH-positive fibers, found around the sigmoid sinus, sent branches to the distal and the intermediate parts of the endolymphatic sac. In these areas. TH-positive fibers traveled freely from blood vessels and formed a loose plexus beneath the lining epithelium. Such fibers were rare in proximal portion of the endolymphatic sac and in the endolymphatic duct. Seven days after elimination of the ipsilateral superior cervical ganglion, some of the TH-positive fibers were gone, however, there were still a few fibers in the endolymphatic sac as well as around the sigmoid sinus, thus their origin remains obscure. CGRP-positive fibers also branched from the fibers around the sigmoid sinus, and were distributed throughout the endolymphatic sac, some occasionally extending to the endolymphatic duct. They not only formed a dense plexus in the sublining space, but also spread through the lining cell layer. Elimination of the ipsilateral superior cervical ganglion did not affect the distribution of CGRP-positive fibers, indicating that they are probably not sympathetic fibers.
采用免疫组织化学技术检测酪氨酸羟化酶（TH）和降钙素基因相关肽（CGRP），以观察10只豚鼠内淋巴囊内交感神经纤维和CGRP阳性纤维的分布情况。在乙状窦周围发现TH阳性纤维网，其分支延伸至内淋巴囊的远端和中间部分。在这些区域，TH阳性纤维从血管自由穿行，在衬里上皮下方形成松散的神经丛。此类纤维在内淋巴囊近端和内淋巴管中较少见。切除同侧颈上神经节后7天，部分TH阳性纤维消失，但内淋巴囊及乙状窦周围仍有少量纤维，其来源尚不清楚。CGRP阳性纤维也从乙状窦周围的纤维发出分支，分布于整个内淋巴囊，部分偶尔延伸至内淋巴管。它们不仅在衬里下间隙形成密集的神经丛，还散布于衬里细胞层。切除同侧颈上神经节不影响CGRP阳性纤维的分布，表明它们可能不是交感神经纤维。
