Haertl R, Bandlow G
Staatliches Medizinal-Untersuchungsamt Osnabrück, Germany.
J Hosp Infect. 1993 Oct;25(2):109-16. doi: 10.1016/0195-6701(93)90101-5.
Eleven multiply resistant Enterobacter cloacae isolates were obtained from eight preterm neonates in a neonatal intensive care unit (NICU) of one hospital in Osnabrück, together with one sensitive strain from another infant. The presence of similar antibiograms and biotypes in 11 isolates prompted further characterization of the isolates by pulsed-field gel electrophoresis (PFGE) of NotI generated genomic restriction fragments. For assessment of the discriminatory power of this typing method 50 non-related strains were included in the study. Non-related strains demonstrated a marked variation in restriction fragment patterns and were clearly discriminated one from another. In contrast, 11 of 12 isolates from the NICU exhibited identical restriction profiles, indicating a cluster associated with nosocomial infection. Since the discriminatory power of PFGE is high, reproducibility is good, and all reagents and equipment for DNA fingerprinting are commercially available, this technique is proposed as a useful tool in the microbiology laboratory for investigating the epidemiology of E. cloacae strains.
从奥斯纳布吕克一家医院新生儿重症监护病房（NICU）的8名早产儿中分离出11株多重耐药阴沟肠杆菌，同时从另一名婴儿身上分离出1株敏感菌株。11株分离株中相似的抗菌谱和生物型促使通过对NotI产生的基因组限制性片段进行脉冲场凝胶电泳（PFGE）对分离株进行进一步鉴定。为评估这种分型方法的鉴别能力，研究中纳入了50株不相关菌株。不相关菌株的限制性片段模式显示出明显差异，能够清晰地区分彼此。相比之下，NICU的12株分离株中有11株表现出相同的限制性图谱，表明存在与医院感染相关的聚集现象。由于PFGE的鉴别能力高、重现性好，且DNA指纹图谱所需的所有试剂和设备均可商购，因此该技术被认为是微生物实验室调查阴沟肠杆菌菌株流行病学的有用工具。
