Dolan S A, Herrfeldt J A, Wellems T E
Laboratory of Malaria Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
Mol Biochem Parasitol. 1993 Sep;61(1):137-42. doi: 10.1016/0166-6851(93)90166-u.
A recombinant DNA clone, pC4.H32, identifies distinguishable restriction fragment patterns from different Plasmodium falciparum clones. Analysis of these DNA fingerprint patterns from parasites cultivated over several years and from progeny of a P. falciparum cross showed the fingerprints to be mitotically and meiotically stable. Restriction fragments from the parents of the cross possessed sufficient polymorphism and number to generate 14 unique fingerprint patterns in 16 independent recombinant progeny. The pC4.H32 insert contains a 0.5-kb imperfectly repeated sequence found in subtelomeric regions of multiple chromosomes. Restriction site variations both within and outside of the 0.5-kb repeat contribute to the fingerprint polymorphisms. Fingerprint analysis can serve to type P. falciparum clones and can detect mislabeling and cross-contamination of parasite stocks.
一个重组DNA克隆pC4.H32能识别不同恶性疟原虫克隆的可区分限制性片段模式。对这些来自多年培养的寄生虫以及恶性疟原虫杂交后代的DNA指纹图谱分析表明，这些指纹图谱在有丝分裂和减数分裂过程中都是稳定的。杂交亲本的限制性片段具有足够的多态性和数量，能够在16个独立的重组后代中产生14种独特的指纹图谱。pC4.H32插入片段包含一个在多条染色体亚端粒区域发现的0.5kb不完全重复序列。0.5kb重复序列内部和外部的限制性位点变异导致了指纹多态性。指纹分析可用于对恶性疟原虫克隆进行分型，并可检测寄生虫株的错误标记和交叉污染。
