Taylor B H, Young R J, Scheuring C F
Department of Biology, Texas A&M University, College Station 77843-3258.
Plant Mol Biol. 1993 Dec;23(5):1005-14. doi: 10.1007/BF00021815.
A cDNA clone corresponding to a transcript that was induced by auxin in tomato seedling roots encodes a 223 amino acid protein belonging to the proteinase inhibitor II family of tomato and potato. The TR8 open reading frame has an N-terminal region with characteristics of a signal peptide and three copies of a 64 amino acid segment that is also present in multiple copies in other PI-II family members. Comparison to other serine proteinase inhibitors indicates that all three domains are trypsin-specific. Transcripts homologous to TR8 increased in abundance within 24 h of auxin treatment and continued to increase through 72 h, at which point the induction was approximately ten-fold. Auxin induction was observed in roots and hypocotyls, but not in cotyledons or epicotyls. Induction may therefore be correlated with lateral and adventitious root initiation, which occurs over a similar time frame in the same tissues. RFLPs detected with the TR8 probe were mapped to the long arm of chromosome 11.
一个与番茄幼苗根中生长素诱导转录本相对应的cDNA克隆编码一种223个氨基酸的蛋白质，该蛋白质属于番茄和马铃薯的蛋白酶抑制剂II家族。TR8开放阅读框具有一个带有信号肽特征的N端区域和一个64个氨基酸片段的三个拷贝，该片段在其他PI-II家族成员中也有多个拷贝。与其他丝氨酸蛋白酶抑制剂的比较表明，所有三个结构域都是胰蛋白酶特异性的。与TR8同源的转录本在生长素处理后24小时内丰度增加，并持续增加至72小时，此时诱导倍数约为10倍。在根和下胚轴中观察到生长素诱导，但在子叶或上胚轴中未观察到。因此，诱导可能与侧根和不定根的起始有关，侧根和不定根在相同组织的相似时间范围内发生。用TR8探针检测到的RFLP被定位到11号染色体的长臂上。
