Shinagawa H, Taniguchi T, Yamaguchi O, Yamamoto K, Honda T
Department of Experimental Chemotherapy, Osaka University, Japan.
Microbiol Immunol. 1993;37(9):689-94. doi: 10.1111/j.1348-0421.1993.tb01693.x.
Enterotoxigenic Escherichia coli strain 260-1 produces colonization factor antigen III and heat-labile enterotoxin. A 55-kb plasmid controlling the expression of the colonization factor antigen was isolated from this strain after it was labeled with ampicillin resistance transposon, Tn3. When this plasmid was introduced into E. coli K-12 strains, it induced the formation of pili that were morphologically and immunologically identical to those on the surface of 260-1 cells, as examined by electron microscopic observation and with the specific antiserum. The physical map of the plasmid was constructed, and the 17.4-kb region was found to be responsible for the expression of the pili.
产肠毒素大肠杆菌菌株260 - 1可产生定植因子抗原III和不耐热肠毒素。在用氨苄青霉素抗性转座子Tn3标记该菌株后，从中分离出一个控制定植因子抗原表达的55 kb质粒。当将该质粒导入大肠杆菌K - 12菌株时，通过电子显微镜观察和使用特异性抗血清检测发现，它诱导形成了与260 - 1细胞表面形态和免疫特性相同的菌毛。构建了该质粒的物理图谱，发现17.4 kb的区域负责菌毛的表达。
