Yamamoto T, Honda T, Miwatani T, Yokota T
J Infect Dis. 1984 Nov;150(5):688-98. doi: 10.1093/infdis/150.5.688.
We studied the colonization-factor antigen I (CFA/I) fimbriae- and heat-stable enterotoxin (ST)-coding plasmid of enterotoxigenic Escherichia coli (strain H10407, serotype O78:H11) pathogenic for humans. With use of conjugal-transfer system of E. coli H10407 and transposon-labeling techniques, the virulence plasmid was shown to be transferable to many species of the family Enterobacteriaceae, including the enteropathogens, Shigella and Salmonella species, and the opportunistic pathogens, Klebsiella, Enterobacter, Citrobacter, Edwardsiella, Serratia, and Proteus species. The virulence plasmid-carrying transconjugants produced both CFA/I fimbriae and ST (exotoxin). Moreover, most of the transconjugants stably inherited the virulence plasmid, although plasmid stability was greatly dependent on culture temperature. Finally, administration of the virulence plasmid-carrying living bacterial cells of Klebsiella, Enterobacter, and Citrobacter species and E. coli K12 resulted in fluid accumulation in both infant-mouse and rabbit ileal-loop tests.
我们研究了对人类致病的产肠毒素大肠杆菌（菌株H10407，血清型O78:H11）的定居因子抗原I（CFA/I）菌毛和热稳定肠毒素（ST）编码质粒。利用大肠杆菌H10407的接合转移系统和转座子标记技术，发现该毒力质粒可转移至肠杆菌科的许多菌种，包括肠道致病菌志贺氏菌和沙门氏菌，以及机会致病菌克雷伯氏菌、肠杆菌、柠檬酸杆菌、爱德华氏菌、沙雷氏菌和变形杆菌。携带毒力质粒的接合子产生CFA/I菌毛和ST（外毒素）。此外，尽管质粒稳定性在很大程度上取决于培养温度，但大多数接合子能稳定遗传毒力质粒。最后，给小鼠和兔回肠环试验中注射携带毒力质粒的克雷伯氏菌、肠杆菌、柠檬酸杆菌和大肠杆菌K12的活细菌细胞，均导致了液体蓄积。
