Bahri-Darwich I, Vaiman D, Olsaker I, Oustry A, Cribiu E P
INRA, Laboratoire de Génétique Biochimique et de Cytogénétique, Centre de Recherches de Jouy, Jouy-en-Josas, France.
Hereditas. 1994;120(3):261-5. doi: 10.1111/j.1601-5223.1994.00261.x.
Two microsatellite-containing cosmids, clOBT361 and clOBT355 were localized to bovine R-banded chromosome 12 and 27, respectively, by fluorescence in situ hybridization. The two microsatellites were subcloned from the cosmids and named INRA209 and INRA206, respectively. Primers were designed from the sequence information and used for PCR amplification with a panel of 36 previously characterized hamster/bovine somatic cell hybrids. This allowed the assignment of the two microsatellites to bovine synteny groups U27 and U8, respectively. The result permits the conclusion that bovine synteny U27 corresponds to BTA12 and that bovine synteny group U8 corresponds to BTA27, reducing to five the number of unassigned bovine synteny groups. Furthermore, as a high level of polymorphism was revealed by the two microsatellites, they have all the required characteristics for good genetic map markers.
通过荧光原位杂交技术，两个含有微卫星的黏粒clOBT361和clOBT355分别被定位到牛的R带染色体12和27上。这两个微卫星从黏粒中被亚克隆出来，分别命名为INRA209和INRA206。根据序列信息设计引物，并用于对一组36个先前已鉴定的仓鼠/牛体细胞杂种进行PCR扩增。这使得这两个微卫星分别被指定到牛的同线群U27和U8。该结果可以得出结论，牛同线群U27对应于牛染色体12（BTA12），牛同线群U8对应于牛染色体27（BTA27），从而使未分配的牛同线群数量减少到5个。此外，由于这两个微卫星显示出高度的多态性，它们具备作为优良遗传图谱标记所需的所有特征。
