Uh S, Lee S M, Kim H T, Chung Y, Kim Y H, Park C, Huh S J, Lee H B
Department of Internal Medicine, Soonchunhyang University, College of Medicine, Seoul, Korea.
Chest. 1994 Jan;105(1):132-7. doi: 10.1378/chest.105.1.132.
The immune response is impaired in patients with malignancy, and radiation therapy (RT) can exacerbate the cancer induced-attenuation of immune response. In order to search for the fine mechanisms behind the RT-induced attenuation of cell-mediated immune response, we measured the number of lymphocytes in peripheral blood, its subsets, and lymphoblast transformation induced by phytohemagglutinin (PHA), purified protein derivatives (PPD), mitogenic monoclonal antibody anti-CD3, and mitogenic combination of anti-CD2 antibodies 9-1 and 9.6 before and after RT in 19 patients with squamous cell lung cancer. Radiation therapy significantly decreased the total numbers of lymphocytes, CD-3, CD-4, and CD8-positive lymphocytes in peripheral blood. However, RT did not change the percentages of lymphocytes and its subsets. Radiation therapy increased the percentage of interleukin 2 (IL-2) receptor-positive lymphocytes, and RT significantly decreased in vitro lymphoblast transformation by PHA, PPD, or monoclonal antibodies to T-cell surface antigens (anti-CD2 or anti-CD3). In vitro incubation with IL-2 did not increase lymphoblast transformation by anti-CD3 before RT but significantly increased after RT. In conclusion, we suggest that one of the fine mechanisms behind the RT-induced suppression of immune responsiveness of patients with lung cancer is a defect in IL-2 synthesis by lymphocytes.
恶性肿瘤患者的免疫反应受损，而放射治疗（RT）会加剧癌症诱导的免疫反应减弱。为了探究RT诱导细胞介导免疫反应减弱背后的精细机制，我们在19例肺鳞状细胞癌患者放疗前后，测量了外周血中淋巴细胞的数量、其亚群以及由植物血凝素（PHA）、纯化蛋白衍生物（PPD）、促有丝分裂单克隆抗体抗CD3和抗CD2抗体9-1与9.6的促有丝分裂组合诱导的淋巴细胞转化情况。放射治疗显著降低了外周血中淋巴细胞、CD-3、CD-4和CD8阳性淋巴细胞的总数。然而，RT并未改变淋巴细胞及其亚群的百分比。放射治疗增加了白细胞介素2（IL-2）受体阳性淋巴细胞的百分比，并且RT显著降低了PHA、PPD或针对T细胞表面抗原的单克隆抗体（抗CD2或抗CD3）诱导的体外淋巴细胞转化。放疗前用IL-2进行体外孵育不会增加抗CD3诱导的淋巴细胞转化，但放疗后会显著增加。总之，我们认为RT诱导肺癌患者免疫反应性抑制背后的精细机制之一是淋巴细胞合成IL-2存在缺陷。
