Ishii T, Ohba M
Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
J Gen Microbiol. 1993 Nov;139(11):2849-54. doi: 10.1099/00221287-139-11-2849.
Seven mosquito-specific strains of Bacillus thuringiensis isolated in Japan were examined for their flagellar (H) antigenicities and the properties of parasporal inclusion proteins. They were assigned to six H serovars: serovar fukuokaensis (H 3ade); serovar canadensis (H 5ac); serovar darmstadiensis (H 10); serovar kyushuensis (H 11ac); serovar shandongiensis (H 22); and an undescribed serovar belonging to H serotype 20. Purified parasporal inclusions exhibited moderate mosquito larvicidal activities with LC50 values ranging from 1 microgram ml-1 to 10 micrograms ml-1. The inclusions of these strains consisted of highly heterogeneous multiple protein components, and five distinct patterns were evident in their SDS-PAGE profiles. Antibodies against kyushuensis inclusion proteins were reactive with all strains to varying degrees, while israelensis antibodies gave only relatively weak reactions with the seven strains. Similarity in antibody-binding profiles was associated with similarity in SDS-PAGE profiles. In a given strain, different antisera gave altered immunoblot profiles. Haemolytic activity was shown by solubilized parasporal inclusion proteins of five of the seven strains.
对在日本分离出的7种苏云金芽孢杆菌的蚊特异性菌株进行了鞭毛（H）抗原性和伴孢晶体蛋白特性的检测。它们被归为6个H血清型：福冈血清型（H 3ade）；加拿大血清型（H 5ac）；达姆施塔特血清型（H 10）；九州血清型（H 11ac）；山东血清型（H 22）；以及一个属于H血清型20的未描述血清型。纯化的伴孢晶体表现出中等程度的杀蚊幼虫活性，LC50值在1微克/毫升至10微克/毫升之间。这些菌株的晶体由高度异质的多种蛋白质成分组成，其SDS-PAGE图谱中有5种明显不同的模式。针对九州菌株晶体蛋白的抗体与所有菌株都有不同程度的反应，而以色列菌株抗体与这7种菌株的反应相对较弱。抗体结合图谱的相似性与SDS-PAGE图谱的相似性相关。在给定的菌株中，不同的抗血清给出了不同的免疫印迹图谱。7种菌株中有5种的伴孢晶体蛋白溶解后表现出溶血活性。
