Ragni A, Thiéry I, Delécluse A
Ecogen Europe, Todi (PG), Italy.
Curr Microbiol. 1996 Jan;32(1):48-54. doi: 10.1007/s002849900009.
Four strains belonging to Bacillus thuringiensis serovars thompsoni, malaysiensis, canadensis, jegathesan and two auto-agglutinating B.t. strains were identified as being highly toxic to the mosquito larvae of the species Aedes aegypti, Anopheles stephensi, and Culex pipiens. Their larvicidal and hemolytic activities were determined and compared with those of strains known to be highly mosquitocidal and/or cytolytic from serovars of B.t. israelensis, morrisoni, darmstadiensis, medellin, kyushuensis, and fukuokaensis. The electrophoretic protein profiles of purified crystals and immunological relationships with B.t.i. polypeptides were studied. Five out of the six new strains showed the same larvicidal and hemolytic activities and the same crystal proteins and toxin genes as B.t.i. One strain, B.t. jegathesan 367, presented a novel pattern of larvicidal activity and a protein profile different from those of other strains.
属于苏云金芽孢杆菌汤普森亚种、马来西亚亚种、加拿大亚种、杰加蒂桑亚种的4个菌株以及2个自凝集苏云金芽孢杆菌菌株被鉴定为对埃及伊蚊、斯氏按蚊和致倦库蚊的幼虫具有高毒性。测定了它们的杀幼虫活性和溶血活性，并与已知来自苏云金芽孢杆菌以色列亚种、莫里森亚种、达姆施塔迪亚种、麦德林亚种、九州亚种和福冈亚种且具有高杀蚊活性和/或细胞溶解活性的菌株进行了比较。研究了纯化晶体的电泳蛋白质图谱以及与以色列亚种多肽的免疫关系。6个新菌株中的5个表现出与以色列亚种相同的杀幼虫活性和溶血活性、相同的晶体蛋白和毒素基因。一个菌株，即杰加蒂桑苏云金芽孢杆菌367，呈现出一种不同于其他菌株的新型杀幼虫活性模式和蛋白质图谱。
