Marklund L, Winterö A K, Thomsen P D, Johansson M, Fredholm M, Gustafsson U, Andersson L
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala.
Anim Genet. 1993 Oct;24(5):333-8. doi: 10.1111/j.1365-2052.1993.tb00336.x.
Restriction fragment length polymorphisms (RFLPs) were described for the porcine loci for beta-glucosidase (GBA) and the beta-polypeptide 1 of the Na+,K(+)-transporting ATPase (ATP1B1). Linkage analyses using a three-generation pedigree provided evidence for the assignment of ATP1B1, GBA and two microsatellite loci (S0001 and S0067) to a previously described linkage group comprising the loci for blood group L (EAL) and an anonymous microsatellite (S0097). The linear order of the six markers was determined with confidence by multipoint analyses and the length of the linkage group was estimated at 88cM. This linkage group was assigned to pig chromosome 4 on the basis of a previous physical localization of the ATP1B1 gene. In situ hybridization data for S0001 presented in this study were consistent with a localization on chromosome 4 and suggested a regional localization to 4p12-p13. The present study reveals conflicting data concerning the genetic localization of the K88 loci controlling the expression of the receptors for the E. coli pilus antigens. One group has reported data suggesting a loose linkage between K88 and EAL, now mapped to chromosome 4, whereas two other groups have found linkage between K88 and the transferrin locus (TF), mapped to chromosome 13 by in situ hybridization.
已描述了猪β-葡萄糖苷酶（GBA）基因座和Na⁺,K⁺-转运ATP酶β多肽1（ATP1B1）的限制性片段长度多态性（RFLP）。利用一个三代家系进行的连锁分析为将ATP1B1、GBA和两个微卫星基因座（S0001和S0067）定位到一个先前描述的连锁群提供了证据，该连锁群包含血型L（EAL）基因座和一个匿名微卫星（S0097）。通过多点分析确定了六个标记的线性顺序，并估计该连锁群的长度为88厘摩（cM）。基于ATP1B1基因先前的物理定位，该连锁群被定位到猪的4号染色体上。本研究中给出的S0001的原位杂交数据与4号染色体上的定位一致，并表明其区域定位在4p12 - p13。本研究揭示了关于控制大肠杆菌菌毛抗原受体表达的K88基因座遗传定位的相互矛盾的数据。一组报道的数据表明K88与EAL之间存在松散连锁，EAL现已定位到4号染色体上，而另外两组发现K88与转铁蛋白基因座（TF）之间存在连锁，TF通过原位杂交定位到13号染色体上。
