猪的K88ab、K88ac大肠杆菌肠道受体与转铁蛋白基因座之间的连锁证据。

Evidence for linkage between K88ab, K88ac intestinal receptors to Escherichia coli and transferrin loci in pigs.

作者信息

Guérin G, Duval-Iflah Y, Bonneau M, Bertaud M, Guillaume P, Ollivier L

机构信息

Laboratoire de Génétique biochimique, INRA-CRJ, Jouy-en-Josas, France.

出版信息

Anim Genet. 1993 Oct;24(5):393-6. doi: 10.1111/j.1365-2052.1993.tb00347.x.

DOI:10.1111/j.1365-2052.1993.tb00347.x

PMID:7904804

Abstract

Segregation at the loci coding for the K88ab and K88ac small intestinal receptors to E. coli adhesins (K88abR, K88acR) and at the transferrin (TF) locus was studied in 38 pig families including 273 piglets. The TF locus showed a segregation deviation towards the B variant while each of the K88 receptors behaved as a single autosomal dominant gene. Recombinants between K88abR and K88acR provide evidence that they are under the control of two different loci. Thirty-two triple backcross families were selected to test linkage and estimate recombination rates (theta). Our results demonstrate that the two K88 receptor loci are closely linked (theta = 0.02) with a maximum lod score value (Zm) of 46.0. In addition, they are linked to the TF locus, theta = 0.14, Zm = 19.6 for the K88abR locus and theta = 0.16, Zm = 17.9 for the K88acR locus. The estimated recombination rates, smaller in males than in females, are consistent with the order TF-K88abR-K88acR. This linkage thus localizes the K88 loci, as the TF locus, on chromosome 13.

摘要

在包括273头仔猪的38个猪家族中，研究了编码大肠杆菌粘附素（K88abR、K88acR）的K88ab和K88ac小肠受体基因座以及转铁蛋白（TF）基因座的分离情况。TF基因座表现出向B变体的分离偏差，而每个K88受体表现为单个常染色体显性基因。K88abR和K88acR之间的重组体提供了它们受两个不同基因座控制的证据。选择了32个三重回交家族来测试连锁关系并估计重组率（θ）。我们的结果表明，两个K88受体基因座紧密连锁（θ = 0.02），最大似然比分数值（Zm）为46.0。此外，它们与TF基因座连锁，K88abR基因座的θ = 0.14，Zm = 19.6，K88acR基因座的θ = 0.16，Zm = 17.9。估计的重组率在雄性中比在雌性中小，这与TF-K88abR-K88acR的顺序一致。因此，这种连锁将K88基因座与TF基因座一样定位在第13号染色体上。