Ertl A P, Gill G N
Department of Medicine, University of California San Diego, La Jolla 92093-0650.
Gene. 1993 Dec 22;136(1-2):361-4. doi: 10.1016/0378-1119(93)90496-p.
The human erbB-2 gene encodes a receptor protein tyrosine kinase which has strong growth-promoting properties. Overexpression of erbB-2 is directly associated with tumor cell growth in vivo and in vitro. To investigate structural features which may contribute to expression, we isolated and sequenced a 3.65-kb 5' promoter fragment of the human erbB-2 gene. This 5' fragment contains an exceptionally high density of four Alu sequences. Other structural features include an A + T-rich region that causes DNA bending, as measured by anomalously slow migration in polyacrylamide gels. These structural features of the 5' region of erbB-2 may contribute to expression of the gene in different growth states.
人类erbB-2基因编码一种具有强大促生长特性的受体蛋白酪氨酸激酶。erbB-2的过表达与体内外肿瘤细胞的生长直接相关。为了研究可能有助于其表达的结构特征，我们分离并测序了人类erbB-2基因的一个3.65 kb的5'启动子片段。这个5'片段包含异常高密度的四个Alu序列。其他结构特征包括一个富含A + T的区域，通过聚丙烯酰胺凝胶中异常缓慢的迁移来测量，该区域会导致DNA弯曲。erbB-2 5'区域的这些结构特征可能有助于该基因在不同生长状态下的表达。
