Borchardt S A, Babwah A V, Jayaswal R K
Department of Biological Sciences, Illinois State University, Normal 61761.
Gene. 1993 Dec 31;137(2):253-8. doi: 10.1016/0378-1119(93)90016-v.
The nucleotide (nt) sequence of a 4.7-kb DNA fragment downstream from a peptidoglycan hydrolase-encoding gene (lytA) from Staphylococcus aureus NCTC8325 was determined. Sequencing revealed three open reading frames (ORFs) of 513, 447 and 879 bp with consensus ribosome-binding sites located upstream from the ATG start codons. Results from in vitro transcription-translation analysis and maxicell experiments suggested that the 447-bp ORF was the one being actively expressed. Comparison of the amino acid (aa) sequences of the ORFs with the aa sequences in the NCBI Entrez database (Release 4.0, April 1993) did not show any significant homology to any sequenced polypeptides. However, nt sequences downstream from lytA showed perfect homology to the bacteriophage phi 11 attachment site (attP) and integration site (attB), and significant homology to downstream regions of the staphylokinase (sak) and exfoliative toxin A (eta) genes of S. aureus.
测定了金黄色葡萄球菌NCTC8325中肽聚糖水解酶编码基因（lytA）下游一个4.7kb DNA片段的核苷酸（nt）序列。测序显示有三个开放阅读框（ORF），长度分别为513、447和879bp，在ATG起始密码子上游有共有核糖体结合位点。体外转录-翻译分析和大细胞实验结果表明，447bp的ORF是正在被活跃表达的那个。将这些ORF的氨基酸（aa）序列与NCBI Entrez数据库（1993年4月第4.0版）中的aa序列进行比较，未显示与任何已测序多肽有任何显著同源性。然而，lytA下游的nt序列与噬菌体phi 11附着位点（attP）和整合位点（attB）显示出完全同源性，并且与金黄色葡萄球菌的葡萄球菌激酶（sak）和剥脱毒素A（eta）基因的下游区域有显著同源性。
