Oshida T, Sugai M, Komatsuzawa H, Hong Y M, Suginaka H, Tomasz A
Rockefeller University, New York, NY 10021.
Proc Natl Acad Sci U S A. 1995 Jan 3;92(1):285-9. doi: 10.1073/pnas.92.1.285.
The Tn551 insertion site of the autolysis-deficient Staphylococcus aureus mutant RUSAL2 was cloned and used to identify the autolysis gene atl in the parent strain, RN450. The open reading frame for atl was 3768 bp in length, encoding a deduced protein of 1256 amino acids and molecular size of 137,381 Da. The atl gene product is a bifunctional protein that has an amidase domain and an endo-beta-N-acetylglucosaminidase domain which must undergo proteolytic processing to generate the two extracellular lytic enzymes found in the culture broth of S. aureus.
自溶缺陷型金黄色葡萄球菌突变体RUSAL2的Tn551插入位点被克隆，并用于鉴定亲本菌株RN450中的自溶基因atl。atl的开放阅读框长度为3768 bp，编码一个推导的1256个氨基酸的蛋白质，分子大小为137,381 Da。atl基因产物是一种双功能蛋白，具有酰胺酶结构域和内切β-N-乙酰氨基葡萄糖苷酶结构域，该结构域必须经过蛋白水解加工才能产生在金黄色葡萄球菌培养液中发现的两种细胞外裂解酶。
