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金黄色葡萄球菌NCTC 8325肽聚糖水解酶基因分析

Analysis of a peptidoglycan hydrolase gene from Staphylococcus aureus NCTC 8325.

作者信息

Wang X, Mani N, Pattee P A, Wilkinson B J, Jayaswal R K

机构信息

Department of Biological Sciences, Illinois State University, Normal 61761.

出版信息

J Bacteriol. 1992 Oct;174(19):6303-6. doi: 10.1128/jb.174.19.6303-6306.1992.

DOI:10.1128/jb.174.19.6303-6306.1992
PMID:1356965
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC207702/
Abstract

We have investigated the expression of the peptidoglycan hydrolase gene (lytA) of Staphylococcus aureus NCTC 8325. Results from in vitro transcription-translation analysis, maxicell experiments, and Northern (RNA) blot analysis suggest that the lytA gene encodes a polypeptide of M(r) approximately 50,000. Physical mapping data indicate that the lytA gene originated from prophage 11 in the NCTC 8325 strain.

摘要

我们研究了金黄色葡萄球菌NCTC 8325肽聚糖水解酶基因(lytA)的表达。体外转录-翻译分析、大细胞实验和Northern(RNA)印迹分析结果表明,lytA基因编码一种分子量约为50,000的多肽。物理图谱数据表明,lytA基因起源于NCTC 8325菌株中的噬菌体11。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7e9/207702/2eea2c6e572d/jbacter00085-0315-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7e9/207702/98b345e30b3e/jbacter00085-0314-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7e9/207702/7d723f4fc1db/jbacter00085-0314-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7e9/207702/2eea2c6e572d/jbacter00085-0315-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7e9/207702/98b345e30b3e/jbacter00085-0314-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7e9/207702/7d723f4fc1db/jbacter00085-0314-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7e9/207702/2eea2c6e572d/jbacter00085-0315-a.jpg

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