Wang X, Mani N, Pattee P A, Wilkinson B J, Jayaswal R K
Department of Biological Sciences, Illinois State University, Normal 61761.
J Bacteriol. 1992 Oct;174(19):6303-6. doi: 10.1128/jb.174.19.6303-6306.1992.
We have investigated the expression of the peptidoglycan hydrolase gene (lytA) of Staphylococcus aureus NCTC 8325. Results from in vitro transcription-translation analysis, maxicell experiments, and Northern (RNA) blot analysis suggest that the lytA gene encodes a polypeptide of M(r) approximately 50,000. Physical mapping data indicate that the lytA gene originated from prophage 11 in the NCTC 8325 strain.
我们研究了金黄色葡萄球菌NCTC 8325肽聚糖水解酶基因(lytA)的表达。体外转录-翻译分析、大细胞实验和Northern(RNA)印迹分析结果表明,lytA基因编码一种分子量约为50,000的多肽。物理图谱数据表明,lytA基因起源于NCTC 8325菌株中的噬菌体11。