一种骨髓移植后限制性片段长度多态性分析的简化快速方法。
A simplified rapid method for restriction fragment length polymorphism analysis after bone marrow transplantation.
作者信息
Schreiner T, Wiesneth M, Maccari B, Sawodny B, Prochnow-Calzia H, Kubanek B
机构信息
Red Cross Blood Center, University of Ulm, Germany.
出版信息
J Immunol Methods. 1994 Feb 10;168(2):183-5. doi: 10.1016/0022-1759(94)90053-1.
PMID:7905898
Abstract
A rapid and simple protocol for restriction fragment length polymorphism (RFLP) analysis using digoxigenin labeled DNA probes is presented. The method described gives consistently good results even with minute cell numbers. The method has been used to evaluate chimerism after bone marrow transplantation but could also find other applications.
摘要
本文介绍了一种使用地高辛标记的DNA探针进行限制性片段长度多态性（RFLP）分析的快速简便方法。所述方法即使对于极少量细胞也能始终产生良好的结果。该方法已用于评估骨髓移植后的嵌合状态，但也可能有其他应用。
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