Borca M V, Kutish G F, Afonso C L, Irusta P, Carrillo C, Brun A, Sussman M, Rock D L
Plum Island Animal Disease Center, Agricultural Research Service, USDA, Greenport, New York 11944.
Virology. 1994 Mar;199(2):463-8. doi: 10.1006/viro.1994.1146.
An open reading frame, LMW8-DR, in the African swine fever virus (ASFV) genome possesses striking similarity to the lymphocyte membrane antigen CD2. All characterized CD2 domains, including the amino-terminal signal sequence, IgV, hinge, IgC2, stalk, transmembrane, and proline-rich carboxy cytoplasmic domains, are highly conserved in the ASFV gene. Critical residues for the binding of the lymphocyte function-associated antigen (LFA-3) and CD59 and for T-cell activation are also partially conserved. LMW8-DR is actively transcribed in ASFV-infected swine macrophages and Vero cells at late times in the infection cycle and Vero and COS cells transiently expressing the LMW8-DR open reading frame hemadsorbed swine red blood cells. The structural and functional similarities of LMW8-DR to CD2, a protein that is involved in cell-cell adhesion and immune response modulation, suggest a possible role in the pathogenesis of ASFV infection.
非洲猪瘟病毒（ASFV）基因组中的一个开放阅读框LMW8-DR与淋巴细胞膜抗原CD2具有显著相似性。所有已鉴定的CD2结构域，包括氨基末端信号序列、IgV、铰链区、IgC2、柄部、跨膜区和富含脯氨酸的羧基胞质结构域，在ASFV基因中高度保守。淋巴细胞功能相关抗原（LFA-3）和CD59结合以及T细胞活化的关键残基也部分保守。LMW8-DR在感染周期后期的ASFV感染猪巨噬细胞和Vero细胞中活跃转录，并且瞬时表达LMW8-DR开放阅读框的Vero和COS细胞会吸附猪红细胞。LMW8-DR与参与细胞间粘附和免疫反应调节的蛋白质CD2在结构和功能上的相似性表明其在ASFV感染发病机制中可能发挥作用。
