Rodríguez J M, Yáñez R J, Almazán F, Viñuela E, Rodriguez J F
Centro de Biología Molecular Severo Ochoa (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid), Facultad de Ciencias, Universidad Autónoma, Spain.
J Virol. 1993 Sep;67(9):5312-20. doi: 10.1128/JVI.67.9.5312-5320.1993.
We have identified an open reading frame, EP402R, within the EcoRI E' fragment of the African swine fever virus genome that encodes a polypeptide of 402 amino acid residues homologous to the adhesion receptor of T cells, CD2. Transcription of EP402R takes place during the late phase of virus replication. The disruption of EP402R, achieved through the replacement of a 354-bp-long fragment from within EP402R by the marker gene lacZ, does not affect the virus growth rate in vitro but abrogates the ability of the virus to induce the adsorption of pig erythrocytes to the surface of infected cells. This result demonstrates that the protein encoded by EP402R is directly involved in the hemadsorption phenomenon induced by the infection of susceptible cells with African swine fever virus.
我们在非洲猪瘟病毒基因组的EcoRI E'片段中鉴定出一个开放阅读框EP402R，它编码一种由402个氨基酸残基组成的多肽，该多肽与T细胞的粘附受体CD2同源。EP402R的转录发生在病毒复制的后期。通过用标记基因lacZ替换EP402R内一个354 bp长的片段实现对EP402R的破坏，这并不影响病毒在体外的生长速度，但消除了病毒诱导猪红细胞吸附到感染细胞表面的能力。这一结果表明，EP402R编码的蛋白质直接参与了非洲猪瘟病毒感染易感细胞所诱导的血细胞吸附现象。
