De Maria A, Colombini S, Schnittman S M, Moretta L
Department of Infectious Diseases, Ospedale S. Martino, Genova, Italy.
Eur J Immunol. 1994 Mar;24(3):531-6. doi: 10.1002/eji.1830240306.
In the present study the requirements for in vitro infection of antigen-specific CD8+ cytotoxic T lymphocytes (CTL) with human immunodeficiency virus -1(HIV-1) were investigated. CD3+CD8+CD4- HIV-1 nef-specific CTL become infected with HIV-1 after short-term co-culture with HLA-matched HIV-1-infected CD20+ B lymphoblastoid cells (B-LCL) which are specifically killed. Similar results were observed with an allospecific CD8+ CTL population. In addition, co-culture experiments showed that once infected with HIV-1, these CD8+ CTL could spread the infection further to uninfected CD4+ lymphocytes. In contrast, CD8+ CTL did not become infected with HIV-1 when co-cultured with HLA-mismatched HIV-1-infected B-LCL which are not killed. These observations in vitro could have relevance in peripheral lymphoid organs contributing to the progressive decrease of HIV-specific CD8+ CTL activity that is associated with the progression to AIDS.
在本研究中，对人免疫缺陷病毒1型（HIV-1）体外感染抗原特异性CD8+细胞毒性T淋巴细胞（CTL）的条件进行了研究。CD3+CD8+CD4-HIV-1 nef特异性CTL在与HLA匹配的、被HIV-1感染的、可被特异性杀伤的CD20+B淋巴母细胞（B-LCL）短期共培养后会被HIV-1感染。在同种特异性CD8+CTL群体中也观察到了类似结果。此外，共培养实验表明，一旦被HIV-1感染，这些CD8+CTL能够将感染进一步传播至未感染的CD4+淋巴细胞。相比之下，当与HLA不匹配的、未被杀伤的、被HIV-1感染的B-LCL共培养时，CD8+CTL不会被HIV-1感染。体外的这些观察结果可能与外周淋巴器官有关，这会导致与艾滋病进展相关的HIV特异性CD8+CTL活性逐渐降低。
