Whetstone C A, Seal B S, Miller J M
Virology Cattle Research, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010.
Vet Microbiol. 1993 Dec;38(1-2):181-9. doi: 10.1016/0378-1135(93)90085-l.
Restriction fragment length polymorphisms (RFLPs) were detected within BHV1.1, BHV1.2, and BHV5 genomes using the restriction enzyme PstI. The genomic areas of these changes has not been previously reported. Using Southern blot hybridization with DNA probes representing the entire genome of BHV1.1, areas of genomic variation were located for a respiratory isolate (BHV1.1), four vaccine isolates (BHV1.1), a genital isolate (BHV1.2), and two encephalitic isolates (BHV5). The most frequently observed RFLPs of BHV1.1 and BHV1.2 occurred within the internal repeat region and the left terminus of the unique long region. When two separate isolates of the encephalitic BHV5 were compared, RFLPs were detected in the internal and right terminal repeat regions. These are the regions of each genome from which immediate early genes are transcribed. No genomic variation was observed throughout the unique long and unique short regions for all BHV1 and 5 isolates examined.
使用限制性内切酶PstI在牛疱疹病毒1.1型（BHV1.1）、牛疱疹病毒1.2型（BHV1.2）和牛疱疹病毒5型（BHV5）基因组中检测到限制性片段长度多态性（RFLP）。这些变化的基因组区域此前尚未见报道。通过使用代表BHV1.1全基因组的DNA探针进行Southern印迹杂交，确定了一株呼吸道分离株（BHV1.1）、四株疫苗株（BHV1.1）、一株生殖道分离株（BHV1.2）和两株脑炎性分离株（BHV5）的基因组变异区域。BHV1.1和BHV1.2中最常观察到的RFLP出现在内部重复区域和独特长区域的左末端。当比较两株独立的脑炎性BHV5分离株时，在内部和右末端重复区域检测到RFLP。这些是每个基因组中立即早期基因转录的区域。在所检测的所有BHV1和BHV5分离株的独特长区域和独特短区域中均未观察到基因组变异。
