Bulach D M, Studdert M J
School of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.
Arch Virol. 1990;113(1-2):17-34. doi: 10.1007/BF01318350.
A clone library of 11 of 15 BamHI fragments representing 81% of the 140 kilobase DNA genome of the prototype bovine encephalitis herpesvirus strain N569 (BEHV.N569) was constructed. The clones were used to verify the BamHI, BstEII, EcoRI, and HindIII genomic maps for BEHV.N569 published by Engels et al. [Virus Res 6: 57-73 (1986)] for the same virus although some amendments/variations to the BamHI map were found in that 3 previously unidentified restriction sites were identified. Restriction site maps for BglII and KpnI were also derived for BEHV.N569. Southern blot analysis using 32P-labelled BEHV DNA as probe indicated that bovine herpesvirus 1 (BHV1), buffalo herpesvirus 1 (BuHV1) and caprine herpesvirus 1 (CaHV1) were similar and that the similarity occurred throughout the entire length of the genomes; CaHV1 was more distantly related to the other 3 viruses. Because of the similarities BEHV.N569 and BHV1. Cooper cloned DNA fragments were used to construct BamHI, BglII, BstEII, EcoRI, KpnI, and HindIII restriction site maps for the genome of BuHV1 and BamHI, BglII, and KpnI maps for the genome of BHV1.V155, a genital strain.
构建了一个克隆文库，该文库包含代表原型牛脑炎疱疹病毒N569株（BEHV.N569）140千碱基DNA基因组81%的15个BamHI片段中的11个。这些克隆用于验证恩格斯等人[《病毒研究》6: 57 - 73(1986)]发表的BEHV.N569的BamHI、BstEII、EcoRI和HindIII基因组图谱，尽管在BamHI图谱中发现了一些修正/变异，即确定了3个先前未识别的限制性酶切位点。还推导了BEHV.N569的BglII和KpnI限制性酶切位点图谱。使用32P标记的BEHV DNA作为探针的Southern印迹分析表明，牛疱疹病毒1型（BHV1）、水牛疱疹病毒1型（BuHV1）和山羊疱疹病毒1型（CaHV1）相似，并且这种相似性存在于整个基因组长度；CaHV1与其他3种病毒的亲缘关系更远。由于BEHV.N569和BHV1存在相似性，库珀克隆的DNA片段被用于构建BuHV1基因组的BamHI、BglII、BstEII、EcoRI、KpnI和HindIII限制性酶切位点图谱以及生殖器毒株BHV1.V155基因组的BamHI、BglII和KpnI图谱。
