Alexander H S, Key D W, Nagy E
Department of Pathobiology, University of Guelph, Ontario.
Can J Vet Res. 1998 Jan;62(1):68-71.
The polymerase chain reaction (PCR) was used to amplify DNA of infectious laryngotracheitis virus (ILTV) isolates obtained from field specimens. The examined 47 samples included 37 isolates representing 35 cases of infectious laryngotracheitis from Ontario and 10 isolates originating from 10 field cases in New Brunswick. The viruses were grown in either embryonated chicken eggs or cell culture, the DNA extracted and amplified using primers designed from the sequence information of a 1.1 kb BamHI fragment of the Ontario 1598 ILTV strain. Thirty-four of the Ontario isolates and all of the New Brunswick isolates were amplified successfully. This suggests that the selected primers would be useful for the majority of the isolates encountered in outbreaks of ILTV.
采用聚合酶链反应（PCR）扩增从现场样本中获得的传染性喉气管炎病毒（ILTV）分离株的DNA。所检测的47个样本包括37个分离株，代表安大略省35例传染性喉气管炎病例，以及10个来自新不伦瑞克省10例现场病例的分离株。病毒在鸡胚或细胞培养物中培养，提取DNA并使用根据安大略省1598 ILTV株1.1 kb BamHI片段的序列信息设计的引物进行扩增。安大略省的34个分离株和新不伦瑞克省的所有分离株均成功扩增。这表明所选择的引物对ILTV疫情中遇到的大多数分离株都有用。
