• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

金黄色葡萄球菌自溶缺陷型突变体：病理学考量、基因定位及电子显微镜研究

Autolysis-defective mutant of Staphylococcus aureus: pathological considerations, genetic mapping, and electron microscopic studies.

作者信息

Mani N, Baddour L M, Offutt D Q, Vijaranakul U, Nadakavukaren M J, Jayaswal R K

机构信息

Department of Biological Sciences, Illinois State University, Normal 61790-4120.

出版信息

Infect Immun. 1994 Apr;62(4):1406-9. doi: 10.1128/iai.62.4.1406-1409.1994.

DOI:10.1128/iai.62.4.1406-1409.1994
PMID:7907580
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC186294/
Abstract

In an earlier report, we had described the isolation and characterization of autolysis-defective mutants of Staphylococcus aureus (N. Mani, P. Tobin, and R.K. Jayaswal, J. Bacteriol. 175:1493-1499, 1993). In the study reported here, an autolysis-defective mutant showed attenuated virulence in a rat model of experimental endocarditis, supporting the role of autolysins in pathogenicity. Transmission electron micrographs of the mutant cells revealed a rough outermost surface as compared with the parent strain, ISP2018. Treatment of mutant cells with lysozyme, proteases, and lipase failed to alter this rough appearance. Physical and genetic data locate the site of mutation between the omega 1100 and ilv loci on the S. aureus chromosome.

摘要

在一份较早的报告中，我们描述了金黄色葡萄球菌自溶缺陷型突变体的分离与特性鉴定（N. 马尼、P. 托宾和R.K. 贾亚斯瓦尔，《细菌学杂志》175:1493 - 1499，1993年）。在此报道的研究中，一个自溶缺陷型突变体在实验性心内膜炎大鼠模型中显示出毒力减弱，这支持了自溶素在致病性中的作用。与亲本菌株ISP2018相比，突变体细胞的透射电子显微镜图像显示其最外层表面粗糙。用溶菌酶、蛋白酶和脂肪酶处理突变体细胞未能改变这种粗糙外观。物理和遗传数据将突变位点定位在金黄色葡萄球菌染色体上的ω1100和ilv基因座之间。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e93/186294/45f7e20f0df4/iai00004-0288-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e93/186294/45f7e20f0df4/iai00004-0288-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e93/186294/45f7e20f0df4/iai00004-0288-a.jpg

相似文献

1
Autolysis-defective mutant of Staphylococcus aureus: pathological considerations, genetic mapping, and electron microscopic studies.金黄色葡萄球菌自溶缺陷型突变体：病理学考量、基因定位及电子显微镜研究
Infect Immun. 1994 Apr;62(4):1406-9. doi: 10.1128/iai.62.4.1406-1409.1994.
2
Molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of Staphylococcus aureus.金黄色葡萄球菌独特自溶基因lytM的分子克隆、测序及表达
J Bacteriol. 1997 Jun;179(11):3625-31. doi: 10.1128/jb.179.11.3625-3631.1997.
3
Isolation and characterization of autolysin-defective mutants of Staphylococcus aureus that form cell packets.形成细胞包块的金黄色葡萄球菌自溶素缺陷突变体的分离与鉴定
Curr Microbiol. 1997 Jun;34(6):354-9. doi: 10.1007/s002849900195.
4
Role of Rot in bacterial autolysis regulation of Staphylococcus aureus NCTC8325.金黄色葡萄球菌 NCTC8325 中 Rot 在细菌自溶调控中的作用。
Res Microbiol. 2013 Sep;164(7):695-700. doi: 10.1016/j.resmic.2013.06.001. Epub 2013 Jun 15.
5
Identification and molecular characterization of an N-acetylmuramyl-L-alanine amidase Sle1 involved in cell separation of Staphylococcus aureus.参与金黄色葡萄球菌细胞分离的N-乙酰胞壁酰-L-丙氨酸酰胺酶Sle1的鉴定及分子特征分析
Mol Microbiol. 2005 Nov;58(4):1087-101. doi: 10.1111/j.1365-2958.2005.04881.x.
6
Isolation and characterization of autolysis-defective mutants of Staphylococcus aureus created by Tn917-lacZ mutagenesis.通过Tn917-lacZ诱变产生的金黄色葡萄球菌自溶缺陷突变体的分离与鉴定。
J Bacteriol. 1993 Mar;175(5):1493-9. doi: 10.1128/jb.175.5.1493-1499.1993.
7
A Staphylococcus aureus autolysin that has an N-acetylmuramoyl-L-alanine amidase domain and an endo-beta-N-acetylglucosaminidase domain: cloning, sequence analysis, and characterization.一种具有N - 乙酰胞壁酰 - L - 丙氨酸酰胺酶结构域和内切β - N - 乙酰葡糖胺酶结构域的金黄色葡萄球菌自溶素：克隆、序列分析及特性鉴定
Proc Natl Acad Sci U S A. 1995 Jan 3;92(1):285-9. doi: 10.1073/pnas.92.1.285.
8
The small RNA SprX regulates the autolysin regulator WalR in Staphylococcus aureus.小 RNA SprX 调控金黄色葡萄球菌自溶素调节子 WalR。
Microbiol Res. 2021 Sep;250:126785. doi: 10.1016/j.micres.2021.126785. Epub 2021 May 5.
9
Identification of sarV (SA2062), a new transcriptional regulator, is repressed by SarA and MgrA (SA0641) and involved in the regulation of autolysis in Staphylococcus aureus.新型转录调节因子sarV（SA2062）的鉴定受到SarA和MgrA（SA0641）的抑制，并参与金黄色葡萄球菌自溶的调节。
J Bacteriol. 2004 Aug;186(16):5267-80. doi: 10.1128/JB.186.16.5267-5280.2004.
10
Expression analysis of the autolysin gene (atl) of Staphylococcus aureus.金黄色葡萄球菌自溶素基因（atl）的表达分析
Microbiol Immunol. 1998;42(9):655-9. doi: 10.1111/j.1348-0421.1998.tb02336.x.

引用本文的文献

1
Staphylococcus aureus sacculus mediates activities of M23 hydrolases.金黄色葡萄球菌囊介导 M23 水解酶的活性。
Nat Commun. 2023 Oct 23;14(1):6706. doi: 10.1038/s41467-023-42506-w.
2
One fold, many functions-M23 family of peptidoglycan hydrolases.一种折叠，多种功能——肽聚糖水解酶的M23家族
Front Microbiol. 2022 Oct 21;13:1036964. doi: 10.3389/fmicb.2022.1036964. eCollection 2022.
3
Cwp19 Is a Novel Lytic Transglycosylase Involved in Stationary-Phase Autolysis Resulting in Toxin Release in .Cwp19 是一种新型溶糖苷酶，参与静止期自溶，导致毒素释放。

本文引用的文献

1
Isolation and characterization of autolysis-defective mutants of Staphylococcus aureus created by Tn917-lacZ mutagenesis.通过Tn917-lacZ诱变产生的金黄色葡萄球菌自溶缺陷突变体的分离与鉴定。
J Bacteriol. 1993 Mar;175(5):1493-9. doi: 10.1128/jb.175.5.1493-1499.1993.
2
Stimulation of spreading of trypsinized human fibroblasts by lysozymes from Staphylococcus aureus, hen egg white, and human urine.金黄色葡萄球菌、鸡蛋清和人尿中的溶菌酶对胰蛋白酶处理过的人成纤维细胞扩散的刺激作用。
In Vitro. 1980 Sep;16(9):738-50. doi: 10.1007/BF02619307.
3
The effect of staphylocoagulase in the mammary gland of the mouse.
mBio. 2018 Jun 12;9(3):e00648-18. doi: 10.1128/mBio.00648-18.
4
Macrophage Polarization Alters Postphagocytosis Survivability of the Commensal Streptococcus gordonii.巨噬细胞极化改变共生链球菌吞噬后的存活能力。
Infect Immun. 2018 Feb 20;86(3). doi: 10.1128/IAI.00858-17. Print 2018 Mar.
5
High Level Expression and Purification of Atl, the Major Autolytic Protein of Staphylococcus aureus.金黄色葡萄球菌主要自溶蛋白Atl的高效表达与纯化
Int J Microbiol. 2014;2014:615965. doi: 10.1155/2014/615965. Epub 2014 Jan 19.
6
Identification and characterization of an autolysin-encoding gene of Streptococcus mutans.变形链球菌自溶素编码基因的鉴定与特性分析
Infect Immun. 2005 Jun;73(6):3512-20. doi: 10.1128/IAI.73.6.3512-3520.2005.
7
Staphylococcus caprae strains carry determinants known to be involved in pathogenicity: a gene encoding an autolysin-binding fibronectin and the ica operon involved in biofilm formation.山羊葡萄球菌菌株携带已知与致病性有关的决定因素：一个编码与自溶素结合的纤连蛋白的基因以及参与生物膜形成的ica操纵子。
Infect Immun. 2001 Feb;69(2):712-8. doi: 10.1128/IAI.69.2.712-718.2001.
8
Characterization of passage-selected vancomycin-resistant Staphylococcus aureus strains of diverse parental backgrounds.不同亲本背景的传代选择耐万古霉素金黄色葡萄球菌菌株的特性分析
Antimicrob Agents Chemother. 2000 Feb;44(2):294-303. doi: 10.1128/AAC.44.2.294-303.2000.
9
Staphylococcal cell wall: morphogenesis and fatal variations in the presence of penicillin.葡萄球菌细胞壁：青霉素存在下的形态发生与致命变异
Microbiol Mol Biol Rev. 1998 Dec;62(4):1371-414. doi: 10.1128/MMBR.62.4.1371-1414.1998.
10
Molecular characterization of a chromosomal determinant conferring resistance to zinc and cobalt ions in Staphylococcus aureus.金黄色葡萄球菌中赋予对锌离子和钴离子抗性的染色体决定因素的分子特征分析
J Bacteriol. 1998 Aug;180(16):4024-9. doi: 10.1128/JB.180.16.4024-4029.1998.
葡萄球菌凝固酶在小鼠乳腺中的作用。
Br J Exp Pathol. 1982 Jun;63(3):336-40.
4
Bacterial surface components and the pathogenesis of infectious diseases.细菌表面成分与传染病的发病机制。
Annu Rev Med. 1981;32:29-43. doi: 10.1146/annurev.me.32.020181.000333.
5
Production of experimental endocarditis by coagulase-negative staphylococci: variability in species virulence.凝固酶阴性葡萄球菌所致实验性心内膜炎：菌种毒力的变异性
J Infect Dis. 1984 Nov;150(5):721-7. doi: 10.1093/infdis/150.5.721.
6
Staphylococcus aureus. The persistent pathogen (first of two parts).金黄色葡萄球菌。持续性病原体（两部分中的第一部分）。
N Engl J Med. 1984 May 24;310(21):1368-73. doi: 10.1056/NEJM198405243102107.
7
Virulence of protein A-deficient and alpha-toxin-deficient mutants of Staphylococcus aureus isolated by allele replacement.通过等位基因置换分离的金黄色葡萄球菌蛋白A缺陷型和α-毒素缺陷型突变体的毒力。
Infect Immun. 1987 Dec;55(12):3103-10. doi: 10.1128/iai.55.12.3103-3110.1987.
8
Biological role of the pneumococcal amidase. Cloning of the lytA gene in Streptococcus pneumoniae.肺炎球菌酰胺酶的生物学作用。肺炎链球菌lytA基因的克隆。
Eur J Biochem. 1987 May 4;164(3):621-4. doi: 10.1111/j.1432-1033.1987.tb11172.x.
9
Variation in the expression of cell wall proteins of Staphylococcus aureus grown on solid and liquid media.在固体和液体培养基上生长的金黄色葡萄球菌细胞壁蛋白表达的变化。
Infect Immun. 1988 May;56(5):1061-5. doi: 10.1128/iai.56.5.1061-1065.1988.
10
Contribution of autolysin to virulence of Streptococcus pneumoniae.自溶素对肺炎链球菌毒力的作用
Infect Immun. 1989 Aug;57(8):2324-30. doi: 10.1128/iai.57.8.2324-2330.1989.