Holodniy M, Mole L, Winters M, Merigan T C
AIDS Research Center, Department of Veterans Affairs Medical Center, Palo Alto, CA 94304.
J Acquir Immune Defic Syndr (1988). 1994 Apr;7(4):363-8.
To determine whether human immunodeficiency virus (HIV) viral load has short term stability, eight clinically stable subjects infected with HIV and having CD4 counts ranging between 10-600/mm3, had blood samples taken at 0800 and 1700 on 3 consecutive days and then weekly at 0800 for 1 month (8-10 observations/subject). Plasma HIV RNA, peripheral blood mononuclear cell (PBMC) proviral DNA, serum p24 antigen levels, and mononuclear cell subsets were measured at each time point. Mean plasma HIV RNA, PBMC HIV DNA, and p24 antigen [both regular and immune complex dissociated (ICD)] levels did not change significantly between mornings and afternoons or on successive days or weeks. CD4+, CD8+, and CD56+ number demonstrated a diurnal variation in those subjects with > 200 CD4 cells/mm3. We conclude that HIV viral load demonstrates short-term stability in clinically stable subjects. This stability has important implications for monitoring HIV disease progression or antiretroviral therapy.
为确定人类免疫缺陷病毒（HIV）病毒载量是否具有短期稳定性，选取了8名临床状况稳定、感染HIV且CD4细胞计数在10 - 600/mm³之间的受试者，在连续3天的上午8点和下午5点采集血样，随后在1个月内每周上午8点采集（每位受试者8 - 10次观察）。在每个时间点测量血浆HIV RNA、外周血单个核细胞（PBMC）前病毒DNA、血清p24抗原水平以及单个核细胞亚群。平均血浆HIV RNA、PBMC HIV DNA以及p24抗原[常规及免疫复合物解离（ICD）后的]水平在上午和下午之间、连续的日子或几周内均无显著变化。在CD4细胞>200/mm³的受试者中，CD4⁺、CD8⁺和CD56⁺细胞数量呈现出昼夜变化。我们得出结论，在临床状况稳定的受试者中，HIV病毒载量具有短期稳定性。这种稳定性对于监测HIV疾病进展或抗逆转录病毒治疗具有重要意义。
