Bieniasz P D, Ariyoshi K, Bourelly M A, Bloor S, Foxall R B, Harwood E C, Weber J N
Department of Genito-Urinary Medicine and Communicable Diseases, St Mary's Hospital Medical School, London, UK.
AIDS. 1993 Jun;7(6):803-6. doi: 10.1097/00002030-199306000-00007.
To determine the relationship between infectious virus titre and proviral copy number in peripheral blood mononuclear cells (PBMC) of infected subjects and to ascertain which, if either, is most closely related to CD4+ cell loss and disease progression.
Cellular HIV-1 viraemia was quantified in 45 infected subjects who had not received antiretroviral therapy using limiting dilution tissue culture infective dose (PBMC TCID) and quantitative polymerase chain reaction (PCR) techniques.
Proviral DNA was detected in 44 (98%) and infectious virus in 38 (82%) of the 45 subjects. Viraemia as measured by both culture and PCR was inversely correlated with patient CD4+ cell count and associated with disease status. Measurement using both techniques correlated with each other (Spearman's rank correlation rho = 0.52; P = 0.0006). The ratio of proviral copies to PBMC TCID ranged from 1:1 1000:1. to > 1000:1.
The ratio of provirus:PBMC TCID was highest when the PBMC TCID was low, and approached unity when PBMC TCID was high. This ratio could be influenced by a variety of factors but did not correlate significantly with patient disease status or CD4+ cell count.
确定感染个体外周血单核细胞（PBMC）中感染性病毒滴度与前病毒拷贝数之间的关系，并确定二者中哪一个（如果有）与CD4 +细胞减少和疾病进展关系最为密切。
采用极限稀释组织培养感染剂量（PBMC TCID）和定量聚合酶链反应（PCR）技术，对45名未接受抗逆转录病毒治疗的感染个体的细胞HIV-1病毒血症进行定量分析。
45名受试者中，44名（98%）检测到前病毒DNA，38名（82%）检测到感染性病毒。通过培养和PCR检测的病毒血症均与患者CD4 +细胞计数呈负相关，并与疾病状态相关。两种技术的测量结果相互关联（Spearman等级相关性rho = 0.52；P = 0.0006）。前病毒拷贝数与PBMC TCID的比例范围为1:1至1000:1，甚至大于1000:1。
当PBMC TCID较低时，前病毒与PBMC TCID的比例最高；当PBMC TCID较高时，该比例接近1。该比例可能受多种因素影响，但与患者疾病状态或CD4 +细胞计数无显著相关性。
