Jenkinson S G, Lawrence R A, Zamora C A, Deneke S M
Department of Medicine, University of Texas Health Science Center at San Antonio.
Am J Physiol. 1994 Feb;266(2 Pt 1):L125-30. doi: 10.1152/ajplung.1994.266.2.L125.
N,N'-bis(2-chloroethyl)-N-nitro-sourea (BCNU) is a potent inhibitor of glutathione reductase (GSSG-Red) activity in both tissues and cells. We examined the effects of treating alveolar type II cells with BCNU and found that a marked decrease in cellular GSSG-Red activity occurred in these cells associated with a time-dependent increase in cellular glutathione (GSH) concentrations. The increase in GSH was not found to be related to changes in cellular gamma-glutamyl transpeptidase activity, gamma-glutamylcysteine synthetase activity, nor increased intracellular transport of cystine. When the BCNU-exposed cells were incubated with hydrogen peroxide to produce oxidant stress, the cells exhibited increased susceptibility to oxidant damage when compared with controls, despite the fact that cellular concentrations of GSH were markedly elevated.
N,N'-双(2-氯乙基)-N-硝基脲(BCNU)是组织和细胞中谷胱甘肽还原酶(GSSG-Red)活性的强效抑制剂。我们研究了用BCNU处理II型肺泡细胞的效果，发现这些细胞中细胞GSSG-Red活性显著降低，同时细胞谷胱甘肽(GSH)浓度随时间依赖性增加。未发现GSH的增加与细胞γ-谷氨酰转肽酶活性、γ-谷氨酰半胱氨酸合成酶活性的变化有关，也与细胞内胱氨酸转运增加无关。当用BCNU处理过的细胞与过氧化氢一起孵育以产生氧化应激时，尽管细胞内GSH浓度显著升高，但与对照组相比，这些细胞对氧化损伤的敏感性增加。
