Rabe K F, Perkins R S, Dent G, Gustmann H, Barnes P J
Department of Thoracic Medicine, National Heart and Lung Institute, London, U.K.
Arzneimittelforschung. 1994 Feb;44(2):166-70.
The effect of sulfonated shale oil fractions on the oxidative burst and the mobilization of intracellular calcium in purified guinea pig peritoneal macrophages was investigated in vitro. Three sulfonated shale oil fractions of varying boiling range, No. 1 = R = 1269 (powder, sodium salt of sulfonated shale oil dark), No. 2 = R 3269 (watery solution, sodium salt of sulfonated shale oil pale, Ichthyol hell), No. 3 = R 2069 (watery solution, ammonium salt of sulfonated shale oil dark, Ichthyol) were investigated. Two of three compounds dose-dependently inhibited leukotriene (LT) B4-induced Ca++ mobilization with complete inhibition at 10 mumol/l. The phorbol ester-stimulated generation of superoxide anion was dose-dependently inhibited by all three compounds with an optimal concentration of 1 to 10 mumol/l and maximal inhibition of 34.7, 39 and 38%. High concentrations of the compounds promoted the release of superoxide anion into the buffer medium. The LTB4-stimulated generation of hydrogen peroxide was also dose-dependently inhibited by all three compounds with maximal inhibition of 34, 40 and 32% with an optimal concentration of 100 mumol/l. Our observations support the hypothesis that sulfonated shale oil fractions are capable of modulating inflammatory responses through inhibition of inflammatory cell functions.
体外研究了磺化页岩油馏分对纯化的豚鼠腹腔巨噬细胞氧化爆发及细胞内钙动员的影响。研究了三种不同沸程的磺化页岩油馏分，1号 = R = 1269（粉末，磺化页岩油深色钠盐），2号 = R 3269（水溶液，磺化页岩油浅色钠盐，鱼石脂黑），3号 = R 2069（水溶液，磺化页岩油深色铵盐，鱼石脂）。三种化合物中的两种呈剂量依赖性抑制白三烯（LT）B4诱导的Ca++动员，在10 μmol/L时完全抑制。三种化合物均呈剂量依赖性抑制佛波酯刺激的超氧阴离子生成，最佳浓度为1至10 μmol/L，最大抑制率分别为34.7%、39%和38%。高浓度的化合物促进超氧阴离子释放到缓冲介质中。三种化合物也均呈剂量依赖性抑制LTB4刺激的过氧化氢生成，最佳浓度为100 μmol/L时最大抑制率分别为34%、40%和32%。我们的观察结果支持以下假设：磺化页岩油馏分能够通过抑制炎症细胞功能来调节炎症反应。
