Yu S J, Lo E S, Cochran E J, Lin D H, Faselis C J, Klawans H L, Carvey P M
Department of Neurological Sciences, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612.
Exp Neurol. 1994 Mar;126(1):15-24. doi: 10.1006/exnr.1994.1038.
We have previously demonstrated that extracts of striatal tissue from patients with Parkinson's disease (PD) increase the survival of dopamine neurons in mesencephalic cultures relative to striatal extracts from control patients. In the present study, ventricular cerebrospinal fluid (vCSF) from patients with PD, Alzheimer's disease (AD), and age-matched controls was similarly assessed. vCSF samples were separated into > 10-kDa and < 10-kDa fractions. Cultures incubated with the > 10-kDa fractions from PD and AD patients contained 73 and 13%, respectively, more tyrosine hydroxylase immunoreactive neurons than cultures incubated with vCSF from age-matched controls. This trophic activity was positively correlated with the trophic activity present in striatal extracts from the same patients. The < 10-kDa vCSF fractions from all patient groups inhibited culture growth. These data suggest that the trophic environment in the striatum is altered in PD and can be successfully monitored in CSF.
我们之前已经证明，与对照患者的纹状体提取物相比，帕金森病（PD）患者的纹状体组织提取物可提高中脑培养物中多巴胺能神经元的存活率。在本研究中，我们对PD患者、阿尔茨海默病（AD）患者以及年龄匹配的对照者的脑室脑脊液（vCSF）进行了类似评估。vCSF样本被分离成分子量大于10 kDa和小于10 kDa的组分。与年龄匹配的对照者的vCSF孵育的培养物相比，用PD和AD患者分子量大于10 kDa的组分孵育的培养物中，酪氨酸羟化酶免疫反应性神经元分别多73%和13%。这种营养活性与同一患者纹状体提取物中的营养活性呈正相关。所有患者组分子量小于10 kDa的vCSF组分均抑制培养物生长。这些数据表明，PD患者纹状体中的营养环境发生了改变，并且可以在脑脊液中成功监测到这种变化。
