Roussakis C, Charrier J, Riou D, Biard J F, Malochet C, Meflah K, Verbist J F
SMAB (Substances Marines à Activité Biologique), Faculté de Pharmacie, B.P., Nantes.
Anticancer Drug Des. 1994 Apr;9(2):119-28.
The inhibitory effect of natural substances of marine origin on the erb-B2 oncogene of a human NSCLC-N6 line was demonstrated in vitro by simultaneous study of the expression of the gene and its product, using respectively an erb-B2 specific probe and an anti-c-erb-B2 polyclonal antibody. Preliminary results indicate inhibition ranging from 17-77% of oncogene expression and from 77-90% of product expression. The fact that substances of this type, with different chemical structures, have the common ability to induce terminal differentiation in an experimental model after irreversible blockade in G1 phase suggests a relationship between the inhibition of certain oncogenes and terminal differentiation.
通过分别使用erb - B2特异性探针和抗c - erb - B2多克隆抗体，同时研究基因及其产物的表达，在体外证明了海洋来源天然物质对人NSCLC - N6细胞系erb - B2癌基因的抑制作用。初步结果表明，癌基因表达的抑制率为17 - 77%，产物表达的抑制率为77 - 90%。这类具有不同化学结构的物质，在G1期不可逆阻滞之后，在实验模型中具有诱导终末分化的共同能力，这一事实表明某些癌基因的抑制与终末分化之间存在关联。
