Schwarting R K, Pei G, Söderström S, Ebendal T, Huston J P
Institute of Physiological Psychology I, University of Düsseldorf, Germany.
Behav Brain Res. 1994 Jan 31;60(1):63-71. doi: 10.1016/0166-4328(94)90064-7.
Previous work has shown that unilateral manipulation of vibrissae in the rat can lead to behavioral asymmetries and to neuronal changes in the basal ganglia: in brief, vibrissae stimulation led to increases in neostriatal dopamine release, whereas unilateral removal of vibrissae led to asymmetries in striatal afferents and to bilateral changes in mesencephalic dopamine mechanisms which were related to the occurrence of behavioral asymmetries and the later recovery therefrom. In the present study, the analysis of neuronal mechanisms possibly affected by vibrissae manipulation was extended to the nerve growth factor and the expression of tyrosine hydroxylase mRNA. Unilateral stimulation or removal of the vibrissae did not lead to significant changes in tissue levels of nerve growth factor in the neostriatum, parietal cortex (including the barrel cortex) or the hippocampus. In contrast, tyrosine hydroxylase mRNA in the substantia nigra and ventral tegmental area was affected by vibrissae removal but not by stimulation, as a bilateral increase in labeling was observed on the level of individual neurons. This effect was only observed in animals tested 4 h after vibrissae removal but not after 10 days. The results are discussed with respect to the interaction of vibrissae function with the basal ganglia, the neurotransmitter dopamine and mechanism of functional recovery.
先前的研究表明，对大鼠触须进行单侧操作会导致行为不对称以及基底神经节的神经元变化：简而言之，触须刺激会导致新纹状体多巴胺释放增加，而单侧去除触须会导致纹状体传入神经的不对称以及中脑多巴胺机制的双侧变化，这些变化与行为不对称的发生及其随后的恢复有关。在本研究中，对可能受触须操作影响的神经元机制的分析扩展到了神经生长因子和酪氨酸羟化酶mRNA的表达。单侧刺激或去除触须并未导致新纹状体、顶叶皮质（包括桶状皮质）或海马体中神经生长因子的组织水平发生显著变化。相比之下，黑质和腹侧被盖区的酪氨酸羟化酶mRNA受到触须去除的影响，但不受刺激的影响，因为在单个神经元水平上观察到标记的双侧增加。这种效应仅在去除触须4小时后测试的动物中观察到，而在10天后未观察到。我们将根据触须功能与基底神经节、神经递质多巴胺以及功能恢复机制之间的相互作用来讨论这些结果。
