Hallett Heather, Churchill Lynn, Taishi Ping, De Alok, Krueger James M
Department of Veterinary & Comparative Anatomy, Pharmacology and Physiology, Sleep and Performance Research Center, Program in Neuroscience, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-6520, USA.
Brain Res. 2010 May 28;1333:48-56. doi: 10.1016/j.brainres.2010.03.048. Epub 2010 Mar 23.
Activity-dependent changes in cortical protein expression may mediate long-term physiological processes such as sleep and neural connectivity. In this study we determined the number of nerve growth factor (NGF)- and interleukin-1beta (IL1beta)-immunoreactive (IR) cells in the somatosensory cortex (Sctx) in response to 2 h of mystacial whisker stimulation. Manual whisker stimulation for 2 h increased the number of NGF-IR cells within layers II-V in activated Sctx columns, identified by enhanced Fos-IR. IL1beta-IR neurons increased within layers II-III and V-VI in these activated columns and IL1beta-IR astrocytes increased in layers I, II-III and V as well as the external capsule beneath the activated columns. These whisker-stimulated increases in the Sctx did not occur in the auditory cortex. These data demonstrate that expression of NGF or IL1beta in Sctx neurons and IL1beta in Sctx astrocytes is, in part, afferent input-dependent.
依赖活动的皮质蛋白表达变化可能介导诸如睡眠和神经连接等长期生理过程。在本研究中，我们确定了响应2小时触须刺激后，体感皮层（Sctx）中神经生长因子（NGF）和白细胞介素-1β（IL1β）免疫反应性（IR）细胞的数量。手动触须刺激2小时增加了激活的Sctx柱中II-V层内NGF-IR细胞的数量，通过增强的Fos-IR来识别这些激活的柱。在这些激活的柱中，II-III层和V-VI层内的IL1β-IR神经元增加，并且I层、II-III层和V层以及激活柱下方的外囊中IL1β-IR星形胶质细胞增加。在听觉皮层中未出现这些触须刺激引起的Sctx增加。这些数据表明，Sctx神经元中NGF或IL1β以及Sctx星形胶质细胞中IL1β的表达部分依赖于传入输入。
