Palaniappan C, Gunasekaran M
Department of Biology, Fisk University, Nashville, Tennessee.
Mycopathologia. 1993 Nov;124(2):69-72. doi: 10.1007/BF01103104.
Specific enzymes of ammonium assimilation were measured in cell-free extracts of Nocardia asteroides grown in a synthetic medium with glutamate as the nitrogen source. Cell-free extracts had active glutamine synthetase (GS) and glutamate synthase (GOGAT) and alanine dehydrogenase (ADH) but glutamate dehydrogenase (GDH) could not be detected in the enzyme preparation. This shows that GS/GOGAT is the major pathway of ammonium assimilation in N. asteroides.
在以谷氨酸作为氮源的合成培养基中培养的星形诺卡氏菌的无细胞提取物中，测定了铵同化的特定酶。无细胞提取物具有活性谷氨酰胺合成酶（GS）、谷氨酸合酶（GOGAT）和丙氨酸脱氢酶（ADH），但在酶制剂中未检测到谷氨酸脱氢酶（GDH）。这表明GS/GOGAT是星形诺卡氏菌中铵同化的主要途径。
