Nesbit E A, Gunasekaran M
Department of Biology, Fisk University, Nashville, TN 37208.
Folia Microbiol (Praha). 1993;38(6):451-5. doi: 10.1007/BF02814394.
The growth and the production of extracellular and intracellular lipases were measured from Nocardia asteroides grown under different cultural conditions. Maximal growth and intracellular and extracellular activities were observed at 3 d after inoculation. Among the tested media, synthetic medium induced maximal growth and extracellular activity, whereas tryptic soy broth induced the maximal intracellular lipase activity. The best carbon and nitrogen sources for growth and lipolytic activity were glucose, fructose, glutamate and nitrate, respectively. The optimal C:N ratio for growth was in the range of 1:4 to 2:3 and for lipase activity the range was 2:3 to 3:2. Anything above or below this range was detrimental to the organism and its enzyme activity. Under the conditions of this study, N. asteroides grew best and had the highest lipase activity when compared to N. brasiliensis and N. caviae.
在不同培养条件下培养星状诺卡氏菌，测定其生长情况以及胞外和胞内脂肪酶的产生。接种后3天观察到最大生长以及胞内和胞外活性。在所测试的培养基中，合成培养基诱导出最大生长和胞外活性，而胰蛋白胨大豆肉汤诱导出最大胞内脂肪酶活性。生长和脂解活性的最佳碳源和氮源分别是葡萄糖、果糖、谷氨酸盐和硝酸盐。生长的最佳碳氮比范围是1:4至2:3，脂肪酶活性的最佳碳氮比范围是2:3至3:2。超出或低于此范围的任何情况都会对该生物体及其酶活性产生不利影响。在本研究条件下，与巴西诺卡氏菌和豚鼠诺卡氏菌相比，星状诺卡氏菌生长最佳且脂肪酶活性最高。
