Jayaraman S, Bennett R B, Gunasekaran M
Department of Biology, Fisk University, Nashville, Tennessee 37208.
Microbios. 1992;70(284-285):151-61.
The growth and production of hydrolytic enzymes such as alpha-amylase, esterase and peroxidase as influenced by the type of media, carbon and nitrogen sources and C:N ratio were monitored in Nocardia asteroides at 37 degrees C. Sabouraud dextrose and the synthetic media yielded maximum growth compared with tryptic soy broth. Among the carbon sources (dextrose, fructose, sucrose, maltose, starch and citrate), monosaccharides supported maximum growth and induced higher alpha-amylase activity but repressed the peroxidase activity. On the other hand, the disaccharides and starch produced less growth but induced maximum esterase and peroxidase activities. Glutamate among the nitrogen sources (nitrate, nitrite, ammonium, hydroxylamine, glutamate and casein) supported maximum growth. Glutamate, nitrate and casein induced alpha-amylase and esterase activities but suppressed peroxidase activity. Nitrite, ammonium and hydroxylamine stimulated peroxidase activity to the maximum but repressed alpha-amylase and esterase activities. Low, medium and high C:N ratios induced maximum peroxidase, esterase and alpha-amylase activities, respectively.
在37摄氏度下，监测了诺卡氏菌中水解酶（如α-淀粉酶、酯酶和过氧化物酶）的生长和产生情况，这些酶受培养基类型、碳源和氮源以及碳氮比的影响。与胰蛋白胨大豆肉汤相比，沙氏葡萄糖培养基和合成培养基产生的生长量最大。在碳源（葡萄糖、果糖、蔗糖、麦芽糖、淀粉和柠檬酸盐）中，单糖支持最大生长并诱导较高的α-淀粉酶活性，但抑制过氧化物酶活性。另一方面，二糖和淀粉产生的生长较少，但诱导最大的酯酶和过氧化物酶活性。在氮源（硝酸盐、亚硝酸盐、铵、羟胺、谷氨酸和酪蛋白）中，谷氨酸支持最大生长。谷氨酸、硝酸盐和酪蛋白诱导α-淀粉酶和酯酶活性，但抑制过氧化物酶活性。亚硝酸盐、铵和羟胺将过氧化物酶活性刺激到最大，但抑制α-淀粉酶和酯酶活性。低、中和高碳氮比分别诱导最大的过氧化物酶、酯酶和α-淀粉酶活性。
