Sekeyová Z, Roux V, Xu W, Rehácek J, Raoult D
Unité des Rickettsies, Faculté de Médecine, CNRS, Marseille, France.
Int J Syst Bacteriol. 1998 Oct;48 Pt 4:1455-62. doi: 10.1099/00207713-48-4-1455.
The name Rickettsia slovaca sp. nov. (type strain is strain B) is proposed for a member of the spotted fever group (SFG) rickettsiae which was isolated from Dermacentor marginatus ticks in Slovakia in 1968, and was recently implicated in human febrile illness. This rickettsia can be phenotypically distinguished from other SFG rickettsiae by microimmunofluorescence serotyping, SDS-PAGE, Western blotting and mAbs. Genotypic differences between R. slovaca and the other SFG representatives can be demonstrated by PCR-RFLP analysis, pulsed-field gel electrophoresis and sequencing of 16S rRNA, gltA and ompA genes.
提议将斯洛伐克立克次氏体新种(模式菌株为B菌株)命名为斑点热群(SFG)立克次体的一个成员,该菌株于1968年从斯洛伐克的边缘革蜱中分离出来,最近被认为与人类发热性疾病有关。通过微量免疫荧光血清分型、SDS-PAGE、蛋白质印迹法和单克隆抗体,这种立克次体在表型上可与其他SFG立克次体区分开来。通过PCR-RFLP分析、脉冲场凝胶电泳以及16S rRNA、gltA和ompA基因测序,可以证明斯洛伐克立克次体与其他SFG代表菌株之间的基因型差异。