Rodríguez-Alvarez E, Abalia I, Bilbao J R, Umaran A, Cisterna R
Departamento de Enfermería, Universidad del País Vasco, Euskal Herriko Unibertsitatea, Leioa, Vizcaya.
Enferm Infecc Microbiol Clin. 1994 Feb;12(2):66-70.
There are several bacterial determinants that contribute to the onset of urinary tract infection by E. coli. The present study focuses on some of the virulence factors considered to be most important, as P fimbriae, the siderophore aerobactin and bacterial capsule, which were studied among 123 uropathogenic E. coli strains isolated from outpatients from the Basque Community.
Virulence factors were detected using Molecular Biology techniques, namely DNA hybridization to specific probes prepared in our laboratory.
When probe pap2, specific for fimbrial adherence was used, 36.5% of the strains showed positive hybridization, and 66 and 73% of the strains hybridized to probes for aerobactin and common capsule region, respectively.
We believe that this technology provides a very useful tool for rapid and easy screening of strains harbouring different virulence factors. Nevertheless, the fact that these methods detect genetic determinants that are not always being expressed must be borne in mind.
有几种细菌决定因素会导致大肠杆菌引起尿路感染。本研究聚焦于一些被认为最重要的毒力因子，如P菌毛、铁载体气杆菌素和细菌荚膜，对从巴斯克自治区门诊患者中分离出的123株尿路致病性大肠杆菌菌株进行了研究。
使用分子生物学技术检测毒力因子，即与我们实验室制备的特异性探针进行DNA杂交。
当使用对菌毛黏附具有特异性的探针pap2时，36.5%的菌株显示阳性杂交，分别有66%和73%的菌株与气杆菌素探针和常见荚膜区域探针杂交。
我们认为这项技术为快速、轻松地筛选携带不同毒力因子的菌株提供了非常有用的工具。然而，必须牢记这些方法检测的是并非总是表达的遗传决定因素这一事实。
