Ciprandi G, Buscaglia S, Albano M, Bertolini C, Truffelli T, Catrullo A, Scordamaglia A, Canonica G W
Department of Internal Medicine-DI.M.I., University of Genoa, Italy.
J Investig Allergol Clin Immunol. 1993 Nov-Dec;3(6):311-4.
Chromones are frequently employed in the treatment of allergic rhinoconjunctivitis and asthma. Following our recent investigations concerning the influence of some antiallergic drugs, such as cromoglycate sodium, steroids, oxatomide and ketotifen (H1 antihistamines), and theophylline, on the immune response, in the present study we analyzed the in vitro effects of a new chromone derivative, nedocromil, on the immune response. To this end, the proliferation of peripheral mononuclear cells (PMNCs) induced by mitogen (PHA) and by CD3, CD2 or CD28 monoclonal antibodies (MAbs) has been studied. Since the effects of nedocromil on immunological parameters are achieved at 10(-7) mol/l, in the experiments herein reported the drug was tested in the cultures at concentrations of 10(-8), 10(-7) and 10(-6) mol/l. Furthermore, the effect of nedocromil was evaluated on the surface expression of the following markers expressed by PMNCs upon activation: ICAM-1 (CD54), LFA-1 and alpha 1-acid glycoprotein (alpha 1-AGP). The results of the present investigation showed no effect of nedocromil on these immunological parameters. These data acquire clinical relevance when related to previous reports showing a depression of the immunological response exerted by other compounds, such as ketotifen, theophylline and steroids.
色酮类药物常用于治疗过敏性鼻结膜炎和哮喘。继我们最近对一些抗过敏药物，如色甘酸钠、类固醇、奥沙米特和酮替芬（H1抗组胺药）以及茶碱对免疫反应的影响进行研究之后，在本研究中，我们分析了一种新的色酮衍生物奈多罗米对免疫反应的体外作用。为此，研究了丝裂原（PHA）以及CD3、CD2或CD28单克隆抗体（MAb）诱导的外周血单核细胞（PMNC）增殖情况。由于奈多罗米在10(-7) mol/l时可对免疫参数产生影响，因此在本报告的实验中，该药物在培养物中的测试浓度为10(-8)、10(-7)和10(-6) mol/l。此外，还评估了奈多罗米对PMNC激活后表达的以下标志物的表面表达的影响：细胞间黏附分子-1（ICAM-1，CD54）、淋巴细胞功能相关抗原-1（LFA-1）和α1-酸性糖蛋白（α1-AGP）。本研究结果表明奈多罗米对这些免疫参数没有影响。当将这些数据与之前显示其他化合物（如酮替芬、茶碱和类固醇）对免疫反应有抑制作用的报告相关联时，这些数据具有临床相关性。
