Hsu M M, Chang Y L, Ko J Y, Chen C L, Kao Y F
Department of Otolaryngology, College of Medicine, National Taiwan University, Taipei, R.O.C.
J Formos Med Assoc. 1994 Feb;93(2):104-9.
The purpose of this study is to clarify the relationship between Epstein-Barr virus (EBV) and tumors of the major salivary gland. EBV DNA was detected by polymerase chain reaction (PCR) in parotid secretions in 56% of patients (n = 32) regardless of the presence of tumors in the parotid gland and in tumor tissues in 19% of surgical specimens (n = 21). By Southern blot-hybridization analysis, we found type A EBV in three patients and type B EBV in one patient. In situ hybridization for EBV genome was positive in surgical specimens of salivary glands in 33% of patients (n = 21). Our results suggest that this virus may not play a role in the pathogenesis of most tumors of the major salivary gland. However, two patients with lymphoepithelial carcinoma were found to have a close relationship to EBV as shown by high titers of antibodies in the sera against EBV related antigens by standard immunofluorescent technique and by the presence of many copies of EBV genome in tumor cells using in situ hybridization.
本研究的目的是阐明爱泼斯坦-巴尔病毒（EBV）与大唾液腺肿瘤之间的关系。无论腮腺中是否存在肿瘤，通过聚合酶链反应（PCR）在56%的患者（n = 32）的腮腺分泌物中检测到EBV DNA，在19%的手术标本（n = 21）的肿瘤组织中也检测到EBV DNA。通过Southern印迹杂交分析，我们在3例患者中发现了A型EBV，在1例患者中发现了B型EBV。33%的患者（n = 21）唾液腺手术标本中EBV基因组的原位杂交呈阳性。我们的结果表明，这种病毒可能在大多数大唾液腺肿瘤的发病机制中不起作用。然而，通过标准免疫荧光技术检测发现，两名淋巴上皮癌患者血清中针对EBV相关抗原的抗体滴度很高，并且通过原位杂交发现肿瘤细胞中存在许多EBV基因组拷贝，这表明这两名患者与EBV关系密切。
