来自大触手放射海葵的一种低分子量细胞毒素的稳定性及与膜毒素的相互作用
Stability of a low molecular mass cytotoxin from the sea anemone Radianthus macrodactylus and membrane-toxin interactions.
作者信息
Zhadan G, Kuznetsova S, Opalikova O, Monastyrnaya M, Zykova T, Emelyanenko V, Villar E, Shnyrov V
机构信息
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino Moscow Region.
出版信息
Biochem Mol Biol Int. 1994 Feb;32(2):331-40.
Differential scanning calorimetry, intrinsic fluorescence and SDS-polyacrylamide-gel-electrophoresis have been applied for the study of the low molecular mass cytotoxin from the sea anemone, Radianthus macrodactylus when in solution and in complexes with phospholipids and erythrocyte membranes. The thermodynamic parameters of toxin denaturation in solution have been obtained. It is shown that one molecule of this toxin withdraws 10 +/- 2 dipalmitoylphosphatidylcholine molecules through cooperative transition. The effect of toxin on dog erythrocytes causes a change in the polypeptide contents of erythrocyte ghosts.
差示扫描量热法、内源荧光法和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳已被用于研究来自大海葵大指海葵的低分子量细胞毒素在溶液中以及与磷脂和红细胞膜形成复合物时的情况。已获得该毒素在溶液中变性的热力学参数。结果表明，该毒素的一个分子通过协同转变摄取10±2个二棕榈酰磷脂酰胆碱分子。毒素对犬红细胞的作用导致红细胞空壳多肽含量发生变化。
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