海葵大指海葵8 kDa细胞毒素不可逆热变性的差示扫描量热研究。
Differential-scanning-calorimetric study of the irreversible thermal denaturation of 8 kDa cytotoxin from the sea anemone Radianthus macrodactylus.
作者信息
Zhadan G G, Shnyrov V L
机构信息
Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Salamanca, Spain.
出版信息
Biochem J. 1994 May 1;299 ( Pt 3)(Pt 3):731-3. doi: 10.1042/bj2990731.
Abstract
A differential-scanning-calorimetric study of the thermal denaturation of a sea-anemone (Radianthus macrodactylus) 8 kDa cytolytic toxin was carried out. The calorimetric traces were found to be irreversible and scan-rate-dependent under the experimental conditions employed. Scan-rate-dependent thermograms were explained in terms of a two-state kinetic model N k -->D, where k is a first-order kinetic constant that changes with temperature as given by the Arrhenius equation, N is the native state of the toxin, and D the denatured one.
摘要
对海葵（大指海葵）8 kDa细胞溶解毒素的热变性进行了差示扫描量热研究。在所采用的实验条件下，发现量热曲线是不可逆的且依赖于扫描速率。依赖于扫描速率的热谱图根据双态动力学模型N⇌k⇌D进行了解释，其中k是一级动力学常数，它随温度变化，如阿累尼乌斯方程所示，N是毒素的天然状态，D是变性状态。
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