Gasser R B, Chilton N B, Hoste H, Stevenson L A
Department of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.
Int J Parasitol. 1994 Apr;24(2):291-3. doi: 10.1016/0020-7519(94)90041-8.
Restriction fragment length polymorphisms (RFLP) of the second internal transcribed spacer (ITS-2) of ribosomal DNA were examined for 6 species of ruminant nematodes; Trichostrongylus colubriformis, T. axei, T. vitrinus, Haemonchus contortus, Teladorsagia circumcincta and Cooperia oncophora. The ITS-2 of each species was amplified by PCR and restricted with the endonucleases DraI, HinfI, RsaI and VspI. Using this approach, the 6 species could be distinguished. No intraspecific variation in RFLP's has been detected so far in the ITS-2 of these species, suggesting that this technique has considerable potential for the identification of eggs of different nematode species in faecal samples.
对6种反刍动物线虫的核糖体DNA第二内部转录间隔区（ITS-2）的限制性片段长度多态性（RFLP）进行了检测，这6种线虫分别为：蛇形毛圆线虫、斧形毛圆线虫、透明毛圆线虫、捻转血矛线虫、环形泰勒虫和具钩库珀线虫。通过聚合酶链反应（PCR）扩增每个物种的ITS-2，并使用限制性内切酶DraI、HinfI、RsaI和VspI进行酶切。采用这种方法，可以区分这6个物种。到目前为止，在这些物种的ITS-2中尚未检测到RFLP的种内变异，这表明该技术在鉴定粪便样本中不同线虫物种的虫卵方面具有很大潜力。
