Ferri D, Liquori G E
Institute of Zoology and Comparative Anatomy, University of Bari, Italy.
Acta Histochem. 1994 Mar;96(1):96-103. doi: 10.1016/S0065-1281(11)80015-2.
Mucous cells and enteroendocrine cells of the pyloric region of the ruin lizard (Podarcis sicula campestris De Betta) have been examined by lectin histochemical and immunohistochemical methods. Binding to five plant lectins (Canavalia ensiformis, Con A; Triticum vulgare, wheat germ, WGL; Lotus tetragonolobus, winged pea, WPL; Glycine max, soybean, SBL; Arachis hypogaea, peanut, PNL) was performed to characterize glycoconjugates in the secretory products of superficial and glandular mucous cells. Lectin histochemistry revealed the presence of N-acetyl-D-galactosamine, D-galactose and N-acetyl-D-glucosamine in the pyloric superficial cells. Mucous glandular cells mainly contained neutral glycoproteins with terminal residues of galactose, N-acetyl-D-glucosamine and N-acetyl-D-galactosamine. These cells did not react with Con A after periodate oxidation-sodium borohydride reduction (Paradoxical Con A staining). In the pyloric glands three different types of endocrine cells were identified immunohistochemically: gastrin-, serotonin- and somatostatin-immunoreactive cells; VIP-, bombesin- or cholecystokinin-immunoreactive cells have not been found in the pyloric mucosa.
采用凝集素组织化学和免疫组织化学方法对意大利壁蜥（Podarcis sicula campestris De Betta）幽门区的黏液细胞和肠内分泌细胞进行了研究。通过与五种植物凝集素（刀豆球蛋白A，Con A；小麦胚芽凝集素，WGL；四角豆凝集素，WPL；大豆凝集素，SBL；花生凝集素，PNL）结合，对表层和腺黏液细胞分泌产物中的糖缀合物进行表征。凝集素组织化学显示幽门表层细胞中存在N-乙酰-D-半乳糖胺、D-半乳糖和N-乙酰-D-葡萄糖胺。黏液腺细胞主要含有带有半乳糖、N-乙酰-D-葡萄糖胺和N-乙酰-D-半乳糖胺末端残基的中性糖蛋白。经高碘酸盐氧化-硼氢化钠还原处理后，这些细胞不与Con A发生反应（反常Con A染色）。在幽门腺中，免疫组织化学鉴定出三种不同类型的内分泌细胞：胃泌素免疫反应性细胞、5-羟色胺免疫反应性细胞和生长抑素免疫反应性细胞；在幽门黏膜中未发现血管活性肠肽、蛙皮素或胆囊收缩素免疫反应性细胞。
