Yang J, Tominaga K, Otori Y, Fukuhara C, Tokumasu A, Inouye S
Laboratory of Integrative Brain Function, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Mol Cell Neurosci. 1994 Apr;5(2):97-102. doi: 10.1006/mcne.1994.1011.
Day-night variation of the preprosomatostatin mRNA level in the suprachiasmatic nucleus (SCN), the site of the circadian pacemaker, was determined in rats maintained under light-dark cycles by semiquantitative Northern blot hybridization of micropunched tissues. Preprosomatostatin mRNA abundance in the SCN showed significant variation during a day with a peak level at around light on and a trough at around 4 h after light off. This time course was essentially identical with that previously found in blinded rats, indicating that environmental light did not produce an immediate effect on the preprosomatostatin mRNA level in the SCN. Moreover, this rhythm in the preprosomatostatin mRNA appeared specific to the SCN, since levels in the cortex and anterior hypothalamus did not oscillate even under light-dark conditions.
通过对微打孔组织进行半定量Northern印迹杂交，在处于明暗周期的大鼠中测定了昼夜节律起搏器视交叉上核（SCN）中前促生长抑素原mRNA水平的昼夜变化。SCN中前促生长抑素原mRNA丰度在一天中表现出显著变化，在光照开启时左右达到峰值水平，在光照关闭后约4小时达到谷值。这个时间进程与之前在失明大鼠中发现的基本相同，表明环境光对SCN中前促生长抑素原mRNA水平没有立即产生影响。此外，前促生长抑素原mRNA中的这种节律似乎是SCN特有的，因为即使在明暗条件下，皮质和下丘脑前部的水平也没有波动。
