Revesz T, Greaves M F, Capellaro D, Murray R K
Br J Haematol. 1976 Dec;34(4):623-30. doi: 10.1111/j.1365-2141.1976.tb03609.x.
Binding of purified cholera toxin to cell surface receptors has been visualized by an indirect immunofluorescence procedure. Normal nucleated cells from blood, bone marrow and lymphoid tissues, express these receptors with the possible exception of erythroid precursors. Cells from patients with chronic lymphoid or myeloid leukaemias have a normal receptor expression but acute leukaemic cells showed a marked deficiency in cholera toxin binding. Insertion of purified Gm ganglioside into membranes of acute leukaemic cells provided cellular binding sites for the toxin.
通过间接免疫荧光法已观察到纯化的霍乱毒素与细胞表面受体的结合。来自血液、骨髓和淋巴组织的正常有核细胞表达这些受体，红系前体细胞可能除外。慢性淋巴细胞白血病或髓细胞白血病患者的细胞具有正常的受体表达，但急性白血病细胞显示出霍乱毒素结合明显不足。将纯化的神经节苷脂插入急性白血病细胞膜可提供毒素的细胞结合位点。
