通过离子冲击从人淋巴母细胞中分离的表面多肽的研究。
Study on the surface polypeptides isolated from human lymphoblastoid ce-ls by ionic shock.
作者信息
Lo E H, Ng M H, Ng W S, Lui S, Ho H C
出版信息
Biochim Biophys Acta. 1976 Oct 22;444(3):863-74. doi: 10.1016/0304-4165(76)90332-9.
PMID:791366
Abstract
Ionic shock treatment in the presence of 10% glycerol is an efficient and selective method for extracting cell surface components from Raji cells and effecting the solubilization of up to 22 polypeptides. The majority of these shock-released polypeptides are accessible to lactoperoxidase radioiodination. Sera from rabbits immunized against these soluble extracts are reactive against Raji cell surface as indicated by indirect membrane immunofluorescence and agglutination assays.
摘要
在10%甘油存在的情况下进行离子休克处理，是一种从Raji细胞中提取细胞表面成分并使多达22种多肽溶解的有效且选择性的方法。这些经休克释放的多肽中的大多数可被乳过氧化物酶放射性碘化。通过间接膜免疫荧光和凝集试验表明，用这些可溶性提取物免疫的兔血清可与Raji细胞表面发生反应。
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